C6745

Human Collagen Type IV

from human cell culture, liquid, 0.3 mg/mL, suitable for cell culture

• CAS Number: 9007-34-5
• UNSPSC Code: 12352202
• NACRES: NA.75
• EC Number: 232-697-4
• MDL number: MFCD00130825
• Biological source: human cell culture
• Form: solution
• Technique(s): cell culture | mammalian: suitable
• Concentration: 0.3 mg/mL
• Mol wt: 170—180 kDa

Properties

• Product Name: Collagen Type IV from human cell culture, Bornstein and Traub Type IV, 0.3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
• biological source: human cell culture
• Quality Segment: 200
• sterility: sterile-filtered
• product line: BioReagent
• form: solution
• mol wt: 170—180 kDa
• packaging: pkg of 1 mL
• concentration: 0.3 mg/mL
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• impurities: HBSAG, none detected, HCV, none detected, HIV-1/HIV-2, none detected
• NCBI accession no.: NM_001845.6
• UniProt accession no.: P02462
• binding specificity: Peptide Source: Laminin; , Peptide Source: Nidogen
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... COL4A1(1282)

Description

General description

Collagen IV, derived from human fibroblasts and epithelial cells in a co-culture system, creates an in vitro ECM (extracellular matrix) and serves as an excellent biological scaffold for three dimensional cell culture.

Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.

COL4A1 (collagen type IV α 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats [1]. It is an important constituent of type IV collagen in the basement membrane . The COL4A1 gene is located on human chromosome 13q34 .

Application

Collagen Type IV from human cell culture has been used in iPSC (induced pluripotent stem cells) culture and differentiation. It has also been used as membrane coatings.[1]

Biochem/physiol Actions

COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. This gene is a major constituent required in basal membrane stability. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke.

Features and Benefits

• This Collagen Type IV product is generated by human fibroblasts and epithelial cells in a co-culture system, which produces an ECM in vitro.
• The Collagen Type IV is then purified biochemically.
• It is sterilized by dialysis in an acetic acid and 0.5% chloroform solution and is supplied sterile at a concentration of ~0.3 mg/ml in 25% acetic acid.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)