The complement is supplied in solution at a concentration of approximately 1 mg/mL, with the lot-specific certificate of analysis providing the exact concentration. Solubility is tested by diluting 0.1 mL of the complement stock solution with 0.2 mL of H2O, resulting in a final volume of 0.3 mL. This dilution ratio yields a complement concentration of approximately 0.33 mg/mL.
C1740
Complement component C1q from human serum
≥95% (SDS-PAGE)
Synonym(s):
C1q from human serum
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About This Item
Form:
liquid
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Pricing and availability is not currently available.
General description
C1q, together with C1r and C1s, in the ratio of 1:2:2, form the C1 complex which is the first component of the classical complement pathway. C1q is composed of 18 polypeptide chains (six A, six B, six C) (MW 460 kDa). All contain an 81-aa collagen-like region composed of (Gly-Xaa-Yaa) repeating sequences close to the N-terminus. Three chains (A1B1C1) form a triple helix with the C-terminus forming the globular heads which may be structurally and functionally distinct domains.
Application
Complement component C1q is an important regulator factor for platelet activation. This has been a topic for research, as platelet-leukocyte aggregates play an important role in inflammatory conditions such as coronary heart disease. In particular, C1q has been shown to inhibit collagen induced aggregation and enhance production of reactive oxygen species (ROS).
Biochem/physiol Actions
C1q deficiency, either because of rare homozygous genetic defect, or transcriptional/translational defects, is almost certainly a cause of systemic lupus erythematosus (SLE). The three C1q subunits are expressed through a novel mechanism of transcriptional synchronization.
Physical form
Supplied as a solution in 10 mM HEPES, 300 mM NaCl, pH 7.2
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This Item | |||
|---|---|---|---|
| assay ≥95% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay ≥85% (SDS-GE) | assay - |
| biological source human | biological source - | biological source human | biological source human serum |
| technique(s) activity assay: suitable | technique(s) - | technique(s) activity assay: suitable | technique(s) activity assay: suitable |
| form liquid | form liquid | form solution | form solution |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| Gene Information human ... C1QA(712), C1QB(713), C1QC(714) | Gene Information - | Gene Information human ... C3(718) | Gene Information human ... C4A(720) |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| C1740-1MG | 04061833467961 |
| C1740-.5MG | 04061833467954 |
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The product is given at a concentration of approx. 1mg/ml. The product specification sheet says that product solubilized is clear, under conditions of 0.1 mL + 0.2 mL H2O. Is the concentration 1 mg/ml related to a volume of 0.1 mL (or 0.3 mL; or other?)?
1 answer-
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I ordered the C1740-1MG with lot#0000356664. I would like to know the protein sequences and the extinction coefficient about this protein?
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This product corresponds to purified 'native' human C1q. Its sequence is not determined in-house. Please refer to published sequences, e.g. in GenBank for UniProt accession nos. P02745, P02746, & P02747.
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what is the size of the protein in WB ? when I test it's size it was approximately 24 KD ( the same size as c1q-c )
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C1q is composed of 18 polypeptide chains (six A, six B, six C). When prepared in denaturing sample buffer, and run on SDS PAGE, the chains will likely dissociate and run at their individual molecular weights. The Uniprot entries for C1qA, C1qB, and C1qC list masses of 26,017, 26,722, and 25,774, respectively.
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Is this product suitable for use in cell cultures?
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This product has not been tested for suitability in cell culture. The end user will need to determine suitability. However, there are multiple publications citing the use of this product in cell culture applications. Please see the link below to review an example:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377823/Helpful?
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Hola, me gustaría saber como se reconstituye el producto para su uso en ensayos. Gracias.
1 answer-
This product is not suitable for use in clinical trials. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.
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What is the concentration of C1740?
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The product is supplied at a concentration of approximately 1 mg/ml. The exact concentration is lot specific and reported on the Certificate of Analysis.
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