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Cholera Toxin B subunit

Name: Cholera Toxin B subunit
Brand: SIGMA

GM1-binding, lyophilized powder

Synonym(s):

CTB

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About This Item

UNSPSC Code:

12352200

PubChem Substance ID:

329774902

NACRES:

NA.77

MDL number:

MFCD02356522

Form:

lyophilized powder

Pricing and availability is not currently available.

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Properties

Product Name

Cholera Toxin B subunit, FITC conjugate, lyophilized powder

conjugate

FITC conjugate

Quality Segment

200

form

lyophilized powder

mol wt

~12 kDa

composition

Protein, ~20% Lowry

extent of labeling

~1.0 mol FITC per mol protein

storage temp.

2-8°C

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

Description

Application

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:

to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat[1]
to stain epididymal sperms of mice[2]
to stain peritoneal macrophages of mice[3]

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide

Analysis Note

Activity measured by ELISA using ganglioside GM₁ coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.

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This Item	C9972	C3741	C9903
Sigma-Aldrich C1655 Cholera Toxin B subunit	Sigma-Aldrich C9972 Cholera Toxin B subunit	Sigma-Aldrich C3741 Cholera Toxin B subunit	Sigma-Aldrich C9903 Cholera Toxin B subunit
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
mol wt ~12 kDa	mol wt ~12 kDa	mol wt pentamer 57 kDa, ~12 kDa	mol wt ~12 kDa
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
composition Protein, ~20% Lowry	composition Protein, ~40% Lowry	composition -	composition Protein, ~5% Lowry
extent of labeling ~1.0 mol FITC per mol protein	extent of labeling -	extent of labeling -	extent of labeling -