Gel images are not provided on a lot-to-lot basis. However, historical images are available below. In this example, the marker appears in the middle two lanes.
B2787
Biotinylated Molecular Weight Marker
mol wt 6,500-180,000 Da
Synonym(s):
protein markers, protein molecular weight markers, protein standards
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.25
eCl@ss:
34058011
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
usage
vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
mol wt
6,500-180,000 Da
packaging
vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
storage temp.
−20°C
Application
For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins.[1] Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli[2] or the Weber and Osborn[3] SDS-PAGE procedures.
Preparation Note
Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.
Other Notes
Contains nine biotinylated proteins (approx 0.1 mg total).
This product contains:
- α2-macroglobulin (equine serum)
- β-galactosidase (E. coli)
- phosphorylase b (rabbit muscle)
- catalase (bovine liver)
- alcohol dehydrogenase (yeast)
- carbonic anhydrase (bovine erythrocyte)
- trypsin inhibitor (soy bean)
- lysozyme (chicken egg)
- aprotinin (bovine lung)
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This Item | |||
|---|---|---|---|
| form lyophilized powder | form liquid | form - | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 100, 200 | Quality Level 200, 400 | Quality Level 100, 200 |
| packaging vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose) | packaging vial of 200 μL | packaging - | packaging - |
| mol wt 6,500-180,000 Da | mol wt - | mol wt 26,600-180,000 Da | mol wt - |
| usage vial sufficient for 200 applications (on 10 cm × 10 cm mini gels) | usage vial sufficient for 400 applications on large gels (16 × 18 cm), vial sufficient for 800 mini-gels (10 × 10 cm) | usage - | usage sufficient for 20 applications (on 10 cm × 10 cm mini gels) |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B2787-5X1VL | 04061833427118 |
| B2787-1VL | 04061833427101 |
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Can I see a picture of this marker? I would like to use it for chemiluminescent wester blotting
1 answer -
The data sheets for Products SDS7B2 and B2787 do not provide a specific recommendation on how long the solutions of these molecular weight marker mixtures can be kept frozen at -20°C. What information can you provide?
1 answer-
Our R&D group recommends that after reconstitution, the solutions should be divided into aliquots and immediately frozen; avoid repeated freeze / thaw cycles. The aliquots should last at least a year at -20°C. Storage at -80°C would even be better, if it is convenient.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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