A3641

Apoferritin from equine spleen

0.2 μm filtered

• CAS Number: 9013-31-4
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00081365
• Form: liquid
• Mol wt: major subunit ML 19,889, minor subunit MH 22,200, native ~481.2 kDa (24 subunits, approx. 20 kDa each)

Properties

• Quality Segment: 200
• sterility: 0.2 μm filtered
• form: liquid
• mol wt: major subunit ML 19,889, minor subunit MH 22,200, native ~481.2 kDa (24 subunits, approx. 20 kDa each)
• color: clear to slightly hazy, solution
• storage temp.: 2-8°C

Description

Application

Apoferritin from equine spleen has been used:

• to determine its partition coefficients in phase systems
• in NaCl for iron loading of cultured cells
• in in situ liquid scanning transmission electron microscope for imaging the interface of biology and nanotechnology

Biochem/physiol Actions

This protein shell of ferritin lacking iron is widely used for the calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis.[1] It is very abundant in beta-cells of the pancreas where it acts as an anti-oxidant.[2] When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to protect the cells from oxidant-mediated cytolysis. [3]

Physical form

Solution in 0.135 M sodium chloride.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves