The specific method used to test this material for protease activity is considered proprietary. However, it is based on the enzymatic cleavage of FITC-Casein. See the link below to review the FITC-Casein Enzymatic Assay to demonstrate the principle of this method.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/353/850/c3777enz.pdf
A3059
Bovine Serum Albumin
heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
CAS Number:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, western blot: suitable
Impurities:
essentially globulin free, protease, none detected
Pricing and availability is not currently available.
biological source
bovine
Quality Level
assay
≥98%
form
lyophilized powder
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable, western blot: suitable
impurities
essentially globulin free, protease, none detected
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected, VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:
This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)
- Protein quantitation (as a standard)
- Immunochemistry (as a blocking agent)
- Cell culture (as a nutrient)
This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
- Enzyme assays
- Protein-based assays
- Protease-sensitive techniques that include:
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)
Application
Bovine Serum Albumin has been used in luminescence assays.
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Heat shock fractionated
- Protease-free
- Essentially globulin-free
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
1 of 1
This Item | |||
|---|---|---|---|
| assay ≥98% | assay ≥96% | assay ≥96% (agarose gel electrophoresis) | assay ≥96% (agarose gel electrophoresis) |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) ELISA: suitable, western blot: suitable | technique(s) ELISA: suitable, blocking: suitable, electrophoresis: suitable, western blot: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A3059-500G | 04061835553259 |
| A3059-5G | 04061833693155 |
| A3059-100G | 04061835553242 |
| A3059-50G | 04061835511686 |
| A3059-200MG | 04061833400173 |
| A3059-10G | 04061835504473 |
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Which method do you use to determine the protease concentration?
1 answer-
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Is Product A3059, Albumin from bovine serum (BSA), acetylated to inactivate nucleases?
1 answer-
A3059 is not acetylated. We do not test for the presence of nucleases. Acetylated BSA products would be B2518 or B8894.
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Is Product A3059, Albumin from bovine serum (BSA), suitable for use as a blocking agent in immunoblotting?
1 answer-
We have not tested A3059 for specific use in immunoblotting as a blocking agent, in quality control. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in blotting experiments, by reducing a potential source of background. The Technical Bulletin states that "Globulin free albumins are suitable for use in applications where no other proteins should be present".
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What is the fatty acid content of Product A3059, Albumin from bovine serum (BSA)? Can it be considered fatty acid free?
1 answer-
The fatty acid content of A3059 is not tested in quality control, and it is not reported on our Certificate of Analysis. We would not refer to this product as "fatty acid free".
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product A3059, Albumin from bovine serum (BSA), suitable for use in ELISA?
1 answer-
We have not tested A3059 for specific use in ELISA. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in ELISA, by reducing a potential source of background.
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Is Product A3059, Albumin from bovine serum (BSA), endotoxin-free?
1 answer-
We have not tested A3059 for endotoxin content. Endotoxin-tested BSA products would include A4919, A9306, A9430, A2934, A9306, A9543, or A3675.
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