Each vial contains a minimum of 1 mg of 5-Azacytidine. The exact quantity may vary from lot to lot and is reported in the Certificate of Analysis. This product is tested for solubility at 5 mg/mL in a 1:1 mixture of acetic acid and water. For use in cell culture, the material may be prepared at 1.2 mg in 10 mL of sterile medium without supplements. The stock solution should be stored in aliquots at -20°C. Avoid repeated freeze/thaw cycles. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a1287#product-documentation
A1287
5-Azacytidine
Hybri-Max™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma
Synonym(s):
4-Amino-1-(β-D-ribofuranosyl)-1,3,5-triazin-2(1H)-one, Ladakamycin
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About This Item
Empirical Formula (Hill Notation):
C8H12N4O5
CAS Number:
Molecular Weight:
244.20
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
206-280-2
Beilstein/REAXYS Number:
620461
MDL number:
Form:
lyophilized powder
Quality level:
Pricing and availability is not currently available.
biological source
synthetic (organic)
Quality Level
grade
Hybri-Max™
sterility
γ-irradiated
product line
BioXtra
form
lyophilized powder
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
mp
226-232 °C (dec.) (lit.)
solubility
acetic acid: water (1:1): 5 mg/mL, clear, colorless
antibiotic activity spectrum
viruses
mode of action
DNA synthesis | interferes
storage temp.
−20°C
SMILES string
NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
InChI
1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
InChI key
NMUSYJAQQFHJEW-KVTDHHQDSA-N
Gene Information
human ... DNMT1(1786), DNMT3A(1788)
General description
Chemical structure: nucleoside
Application
Used to study the effects of the DNA incorporated molecule on DNA methylation processes.
5-Azacytidine may be used to induce in vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. It may also be used to study the effects of 5-Azacytidine on the immune regulatory myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs).
Biochem/physiol Actions
A potent growth inhibitor and cytotoxic agent; inhibits DNA methyltransferase, an important regulatory mechanism of gene expression, gene activation and silencing.
Potent growth inhibitor and cytotoxic agent; inhibits DNA methyltransferase.
5-Azacytidine is a deoxycytidine analog and a demethylating agent. It acts as a potential antineoplastic agent for acute myelogenous leukemia.[1] 5-Azacytidine activates repressed genes by inhibiting DNA methylation. 5-Azacytidine also affects protein synthesis by altering the RNA function and stability.[1]
Preparation Note
Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500ml medium. Final working concentration: 10 μM 5-azacytidine.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| form lyophilized powder | form powder | form - | form powder |
| technique(s) cell culture | hybridoma: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 300 | Quality Level - | Quality Level 200 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| solubility acetic acid: water (1:1): 5 mg/mL, clear, colorless | solubility - | solubility - | solubility acetic acid: water (1:1): 50 mg/mL |
| biological source synthetic (organic) | biological source - | biological source - | biological source - |
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Find documentation for the products that you have recently purchased in the Document Library.
5-azacytidine treatment reorganizes genomic histone modification patterns
Komashko VM and Farnham PJ
Epigenetics, 5(3), 229-240 (2010)
DNA methyltransferase inhibitors for cancer therapy
Epigenetics, 5(3), 265-290 (2015)
In vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. The role of 5-azacytidine
Antonitsis P, et al.
Interactive Cardiovascular and Thoracic Surgery, 6(5), 593-597 (2007)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A1287-1VL | 04061833342046 |
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What is the vial content of A1287, what is the solubility, and what are to storage conditions for the prepared stock solution?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the concentration of Product A1287, 5-Azacytidine - Hybri-Max™?
1 answer-
The working concentration is 10 μM, so the 50X stock is 500 μM.
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