If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
93773
TWEEN® 20
viscosity 250-450 mPa.s (25 °C)
Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
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| Pack Size | SKU | Availability | Price |
|---|
About This Item
description
non-ionic
Quality Level
form
viscous liquid
mol wt
~1228
technique(s)
DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable
refractive index
n20/D 1.468 (lit.), n20/D 1.471
viscosity
250-450 mPa.s(25 °C)
HLB value
16.7±1.0
density
1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.)
SMILES string
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
General description
Application
- as an emulsifying agent for the preparation of stable oil-in-water emulsions
- in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
- as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
- for lysing mammalian cells at a concentration of 0.005 to 0.5%
- for rehydrating tissue before in situ hybridization
Biochem/physiol Actions
Features and Benefits
- Suitable for protein purification and DNA and RNA extraction
- Suitable for mammalian cell culture
- High purity product for research applications
Other Notes
Legal Information
Disclaimer
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) DNA extraction: suitable, cell culture | mammalian: suitable, RNA extraction: suitable, protein purification: suitable | technique(s) cell culture | mammalian: suitable | technique(s) blocking: suitable, cell culture | mammalian: suitable, hybridization: suitable, protein purification: suitable, transformation: suitable | technique(s) - |
| form viscous liquid | form viscous liquid | form viscous liquid | form viscous liquid |
| density 1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.) | density 1.095 g/mL at 25 °C (lit.), 1.1 g/cm3 at 25 °C (lit.) | density 1.095 g/mL at 25 °C (lit.) | density 1.095 g/mL at 25 °C ((lit.)), 1.095 g/mL at 25 °C (lit.) |
| mol wt ~1228 | mol wt ~1228 | mol wt ~1228 | mol wt ~1228 |
| viscosity 250-450 mPa.s(25 °C) | viscosity - | viscosity - | viscosity - |
| description non-ionic | description non-ionic | description non-ionic | description non-ionic |
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Storage Class
10 - Combustible liquids
flash_point_f
527.0 °F - Pensky-Martens closed cup
flash_point_c
275 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 93773-250G | 04061833283202 |
| 93773-10ML-KC | 04061838086136 |
| 93773-1KG | 04061833283196 |
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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