The emission is at 570 nm when dissolved in water.
83200
Pyronin Y
suitable for microscopy
Synonym(s):
Pyronin G, Pyronin J
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About This Item
Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
Molecular Weight:
302.80
EC Number:
202-147-8
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3795789
Colour Index Number:
45005
Pricing and availability is not currently available.
mp
250-260 °C (lit.)
solubility
water: 0.01 g/10 mL, red
εmax
≥1650 at 543-553 nm in 50% ethanol, ≥650 at 249-259 nm in 50% ethanol
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
InChI
1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
InChI key
INCIMLINXXICKS-UHFFFAOYSA-M
Biochem/physiol Actions
Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for microscopy | suitability - | suitability suitable for nucleic acid staining | suitability suitable for microscopy |
| solubility water: 0.01 g/10 mL, red | solubility - | solubility water: 10 mg/mL, dark red | solubility H2O: 0.1 g/10 mL |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| mp 250-260 °C (lit.) | mp 250-260 °C (lit.) | mp 250-260 °C (lit.) | mp - |
| εmax ≥1650 at 543-553 nm in 50% ethanol | εmax - | εmax - | εmax ≥350 at 283-293 nm in water, ≥450 at 407-417 nm in water |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Rost FWD
Fluorescence Microscopy (1995)
Karsten Kuritz et al.
Scientific reports, 10(1), 3619-3619 (2020-02-29)
Modern cytometry methods allow collecting complex, multi-dimensional data sets from heterogeneous cell populations at single-cell resolution. While methods exist to describe the progression and order of cellular processes from snapshots of such populations, these descriptions are limited to arbitrary pseudotime
Myron Nevins et al.
Journal of periodontology, 83(7), 878-884 (2012-01-10)
This study tests the effectiveness of hydroxyapatite and collagen bone blocks of equine origin (eHAC), infused with recombinant human platelet-derived growth factor-BB (rhPDGF-BB), to augment localized posterior mandibular defects in non-human primates (Papio hamadryas). Bilateral critical-sized defects simulating severe atrophy
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 83200-10G | 04061833005446 |
| 83200-5G | 04061833005453 |
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What is the emission wavelength of Pyronin Y, Product 83200?
1 answer-
Helpful?
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Has Pyronin Y, Product 83200, been use-tested for the demonstration of RNA?
1 answer-
No. There are, however, two other Pyronin products from Sigma-Aldrich which should be suitable. Product No. P9172 has been used to stain RNA in polyacrylamide gels. Product No. 213519 was submitted to the Biological Stain Commission for certification. Testing was performed by the Biological Stain Commission to ensure the Pyronin Y stained RNA in tissue sections.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the solubility of Pyronin Y, Product 83200, in water and ethanol?
1 answer-
Solubility is not tested at Sigma Aldrich, but the literature reports solubility up to 9% (i.e., 90 mg/mL) in water and 0.6% (6 mg/mL) in ethanol.
Helpful?
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Is there a prepared solution of Methyl Green and Pyronin Y for the demonstration of plasma cells and RNA in tissue sections?
1 answer-
Yes, product HT70116 is available in pre-made form. The product is approved for in vitro diagnostic (IVD) use.
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