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75259

Tris(2-carboxyethyl)phosphine hydrochloride

BioUltra, ≥98% (NMR)

Synonym(s):

TCEP

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About This Item

Empirical Formula (Hill Notation):
C9H15O6P · HCl
CAS Number:
51805-45-9
Molecular Weight:
286.65
UNSPSC Code:
12352128
NACRES:
NA.25
PubChem Substance ID:
329766294
MDL number:
MFCD00145469
Beilstein/REAXYS Number:
3724376
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product line

BioUltra

Quality Level

300

assay

≥98% (NMR)

form

crystals

reaction suitability

reagent type: reductant

loss

≤1.0% loss on drying

pH

1.2-1.5

solubility

H2O: 1 M, clear, colorless (no residue)

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.05 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: 0.05, λ: 280 nm Amax: 0.03

storage temp.

2-8°C

SMILES string

Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

InChI key

PBVAJRFEEOIAGW-UHFFFAOYSA-N

General description

Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution.[1][2] It readily and stoichiometrically reduces disulfides with high specificity.[1] TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.[2]

Application

Tris(2-carboxyethyl)phosphine hydrochloride has been used:
  • as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA[3]
  • in reducing sulfhydryl groups to obtain total thiol fraction[4]
  • to reduce protein samples[5]

Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

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This Item
68957580560C4706
Tris(2-carboxyethyl)phosphine hydrochloride BioUltra, ≥98% (NMR)

Sigma-Aldrich

75259

Tris(2-carboxyethyl)phosphine hydrochloride

Tris(2-carboxyethyl)phosphine hydrochloride BioUltra, suitable for electrophoresis, SDS-PAGE tested

Sigma-Aldrich

68957

Tris(2-carboxyethyl)phosphine hydrochloride

TCEP, Hydrochloride A water-soluble and odorless disulfide-reducing agent that is more stable and effective than DTT.

Millipore

580560

TCEP, Hydrochloride

Tris(2-carboxyethyl)phosphine hydrochloride powder

Sigma-Aldrich

C4706

Tris(2-carboxyethyl)phosphine hydrochloride

assay

≥98% (NMR)

assay

97.5-102.5%

assay

≥98% (elemental analysis)

assay

-

Quality Level

300

Quality Level

100

Quality Level

100

Quality Level

300

product line

BioUltra

product line

BioUltra

product line

-

product line

-

pH

1.2-1.5

pH

-

pH

-

pH

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

form

crystals

form

powder

form

crystalline solid

form

powder

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pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP5030
BCCP4097
BCCN4925
BCCN4923
BCCM1028

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G Elif Karagöz et al.
Bio-protocol, 9(14), e3307-e3307 (2019-07-20)
The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage
Yasunori Sato et al.
Biological & pharmaceutical bulletin, 33(3), 364-369 (2010-03-02)
Ascorbic acid (AA) has a strong anti-oxidant function evident as its ability to scavenge superoxide radicals in vitro. Moreover, AA is an essential ingredient for post-translational proline hydroxylation of collagen molecules. Dehydroascorbic acid (DHA), the oxidized form of AA, is
Lucas B Harrington et al.
Molecular cell, 79(3), 416-424 (2020-07-10)
CRISPR-Cas12c/d proteins share limited homology with Cas12a and Cas9 bacterial CRISPR RNA (crRNA)-guided nucleases used widely for genome editing and DNA detection. However, Cas12c (C2c3)- and Cas12d (CasY)-catalyzed DNA cleavage and genome editing activities have not been directly observed. We
View All Related Papers

Global Trade Item Number

SKUGTIN
343366-25G04061833554531
343366-5G04061833554548
75259-10G04061832907413
75259-1G04061832907420

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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