If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
50950
Guanidine hydrochloride
≥98%
Synonym(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
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About This Item
Quality Segment
assay
≥98%
form
powder or crystals
storage condition
(Tightly closed. Dry. )
color
colorless to white
pH
(25 °C, 4.6 - 6/573 g/L)
mp
180-185 °C (lit.)
solubility
H2O: 6 M, clear (100 Hazen)
density
1.3 g/cm3 (lit.)
SMILES string
Cl[H].NC(N)=N
InChI
1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
InChI key
PJJJBBJSCAKJQF-UHFFFAOYSA-N
Gene Information
human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)
General description
At lower concentrations, guanidine hydrochloride has the intriguing ability to promote the refolding of denatured proteins, aiding in protein renaturation studies. In RNA extraction, it acts as a strong denaturant, disrupting cell structures and ensuring the integrity of extracted RNA by inactivating RNA enzymes. Overall, guanidine hydrochloride′s denaturing and renaturing properties make it an essential reagent for various cell biology applications, including protein purification, nucleic acid isolation, and protein refolding studies.
Application
- for lysing homogenized brain tissue
- in the preparation of incubation buffer for Ni-sepharose protein binding, purification, propionylation, and on-bead digestion to minimize nonspecific binding to the affinity resin
- in the preparation of solutions/extraction buffer to extract soluble protein from the human tissue
Biochem/physiol Actions
Features and Benefits
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| assay ≥98% | assay ≥99% | assay ≥99% (titration) | assay ≥99.0% (AT) |
| Quality Level 200 | Quality Level 400 | Quality Level 200, 400 | Quality Level 200 |
| form powder or crystals | form crystalline powder | form crystalline powder | form solid |
| solubility H2O: 6 M, clear (100 Hazen) | solubility H2O: 6 M, clear, colorless | solubility H2O: 6 M | solubility H2O: 6 M at 20 °C, clear, colorless |
| density 1.3 g/cm3 (lit.) | density 1.3 g/cm3 (lit.) | density 1.3 g/cm3 (lit.) | density 1.3 g/cm3 (lit.) |
| pH (25 °C, 4.6 - 6/573 g/L) | pH (25 °C, 4.6 - 6/573 g/L) | pH (25 °C, 4.6 - 6 at 573 g/L) | pH (25 °C, 4.6 - 6/573 g/L) |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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