41785

Dimidium bromide

BioReagent, suitable for fluorescence, ~95% (AT)

• Synonym(s): 3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide, Trypadine, phenanthridinium 1553
• Empirical Formula (Hill Notation): C₂₀H₁₈BrN₃
• CAS Number: 518-67-2
• Molecular Weight: 380.28
• UNSPSC Code: 12171500
• NACRES: NA.32
• PubChem Substance ID: 57650090
• EC Number: 208-256-7
• Beilstein/REAXYS Number: 3809779
• MDL number: MFCD00011757
• Assay: ~95% (AT)
• Form: solid

Properties

• product line: BioReagent
• Quality Segment: 100
• assay: ~95% (AT)
• form: solid
• mol wt: 380.28 g/mol
• concentration: 80-100 % (w/w) (3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide)
• color: dark purple to very dark purple, dark red to very dark red
• mp: 243-248 °C (lit.)
• fluorescence: λ_ex 306 nm; λ_em 612 nm in 50 mM Tris pH 8.0 (DNA), λ_ex 480 (pH 7.6, low ionic strength)
• suitability: suitable for fluorescence
• SMILES string: [Br-].C[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• InChI: 1S/C20H17N3.BrH/c1-23-19-12-15(22)8-10-17(19)16-9-7-14(21)11-18(16)20(23)13-5-3-2-4-6-13;/h2-12,22H,21H2,1H3;1H
• InChI key: MQOKYEROIFEEBH-UHFFFAOYSA-N

Description

General description

Dimidium Bromide is a trypanocidal drug that is known to affect nucleic acid synthesis in many organisms e.g. tissue culture cells.[1]

Application

Dimidium Bromide is used as a binding agent to natural DNA based on base-pair specificity.[2] It is used as a fluorescence agent for nucleic acid experiments.

Intercalating probe for nucleic acids.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves