Giemsa solution should never be diluted in PBS. Giemsa solution is typically diluted with either deionized water or a low molarity phosphate buffer. Weise and Sorsensen phosphate buffer solutions are typically used. Dilutions can range from 1:10 to 1:80. The most common range for dilutions are either 1:20 or 1:40.
32884
Giemsa Stain
Modified Solution
Synonym(s):
Azure eosin methylene blue, Giemsa solution
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About This Item
CAS Number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
MA.02
MDL number:
Pricing and availability is not currently available.
Product Name
Giemsa Stain, Modified Solution, according to Giemsa
form
liquid
Quality Level
quality
according to Giemsa
technique(s)
UV/Vis spectroscopy: suitable
color
dark blue
pH
6.9
density
1.036 g/cm3 at 20 °C
suitability
passes test for UV spectrum
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
General description
Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.[1]
Application
Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.[1]
Biochem/physiol Actions
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
Other Notes
Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.
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This Item | |||
|---|---|---|---|
| technique(s) UV/Vis spectroscopy: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - |
| suitability passes test for UV spectrum | suitability - | suitability - | suitability - |
| color dark blue | color - | color - | color - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) hematology | application(s) hematology |
| form liquid | form - | form solution | form solution |
| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. room temp |
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
target_organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 2
flash_point_f
51.8 °F - closed cup
flash_point_c
11 °C - closed cup
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Xiao Liu et al.
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S R Patil et al.
Science (New York, N.Y.), 173(3999), 821-822 (1971-08-27)
Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the
Yonglian Zeng et al.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 32884-1L | 04061826717905 |
| 32884-250ML | 04061826717912 |
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To stain a blood smear does this product need to be diluted in PBS? What is the recommended ratio of stain to PBS?
1 answer-
Helpful?
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Hello I would like to ask if this product is suitable for G banding of human chromosomes. Thank you very much!
1 answer-
Product 32884 has not been suitability tested for G banding of human chromosomes. There is no available protocol for use of the product for G banding of human chromosomes. The quality control testing performed on a lot-to-lot basis includes testing for appearance, density, pH, and UV absorbance.
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