26745
Cholesterol Esterase from porcine pancreas
lyophilized, powder, white, ~35 U/mg
Synonym(s):
bile salt-dependent lipase, bile salt-stimulated lipase, carboxyl ester lipase, nonspecific lipase, pancreatic lysophospholipase, Cholesterol Esterase from hog pancreas, Sterol-ester acylhydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.77
EC Number:
232-808-6
MDL number:
Specific activity:
~35 U/mg
Biological source:
Porcine pancreas
Pricing and availability is not currently available.
biological source
Porcine pancreas
Quality Level
form
powder
quality
lyophilized
specific activity
~35 U/mg
mol wt
Mr ~440000
color
white
storage temp.
−20°C
General description
Research area: Cell signaling
Pancreatic cholesterol esterase (CEase), also known as bile salt stimulated lipase, is a serine hydrolase belonging to the α/β hydrolase family of enzymes. They are released from the exocrine pancreas. Serine 194, histidine 435 and aspartate 320 represent the catalytic triad of the enzyme.
Pancreatic cholesterol esterase (CEase), also known as bile salt stimulated lipase, is a serine hydrolase belonging to the α/β hydrolase family of enzymes. They are released from the exocrine pancreas. Serine 194, histidine 435 and aspartate 320 represent the catalytic triad of the enzyme.
Application
Cholesterol Esterase from porcine pancreas has been used:
- in an in vitro simulated digestion model to improve the hydrolysis of carotenoid esters in the intestinal phase[1]
- to analyze the bioavailability of phytosterol and cholesterol in the aqueous micellar phase of an in vitro simulated digestion model[2]
- as a standard in enzyme activity assay[3]
Biochem/physiol Actions
Cholesterol esterase (CEase), an enzyme having broad substrate specificity plays a key role in regulating the bile salt mediated hydrolysis of dietary cholesteryl esters. It also participates in the hydrolysis of triglycerides and phospholipids which are essential for normal cholesterol absorption.[4] Cholesterol esterase hydrolysis helps in the determination of total cholesterol levels in the serum and plasma. Additionally, cholesterol esterase (CEase) along with phospholipase A2 is involved in the hydrolysis of lecithin to lysolecithin, forming intestinal micelles that deliver free cholesterol to enterocytes. Loss of enzyme function results in reduced intestinal absorption of dietary cholesteryl esters and prevents the formation of intestinal micelles, thereby failing to deliver cholesterol efficiently.[4] Circulating cholesterol esterases, accumulated in atherosclerotic lesions are involved in triggering the proliferation of smooth muscle cells. Lastly, they are also selectively involved in the hydrolysis/condensation of carboxylic ester bonds.
Other Notes
1 U corresponds to the amount of enzyme which liberates 1 μmol cholesterol per minute at pH 7.0 and 37°C (cholesterol acetate as substrate)
Disclaimer
Sales restrictions may apply.
1 of 1
This Item | |||
|---|---|---|---|
| biological source Porcine pancreas | biological source - | biological source Porcine liver | biological source Porcine pancreas |
| specific activity ~35 U/mg | specific activity ≥200 units/g protein | specific activity ≥50 U/mg | specific activity ≥4.0 units/mg protein (biuret) |
| form powder | form lyophilized powder | form lyophilized solid, powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt Mr ~440000 | mol wt 84 kDa by SDS-PAGE | mol wt Mr ~162000 | mol wt - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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R.J. Kazlauskas
Journal of the American Chemical Society, 111, 4953-4953 (1989)
Baojian Li et al.
European journal of medicinal chemistry, 45(5), 1955-1963 (2010-02-13)
Due to the importance of pancreatic cholesterol esterase (CEase) as a potential target in atherosclerosis and for the development of hypocholesterolemic agents, there are increasing interests in designing and synthesizing CEase inhibitors. In the present study, we prepared forty-five isocoumarin
Isocoumarin-based inhibitors of pancreatic cholesterol esterase
Heynekamp JJ, et al.
Journal of Separation Science, 16(9), 5285-5294 (2008)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 26745-10MG-F | 04061826134306 |
| 26745-100MG-F | 04061833616185 |