21879

5(6)-Carboxyfluorescein diacetate

BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

• Synonym(s): 5(6)-Carboxy-di-O-acetylfluorescein
• Empirical Formula (Hill Notation): C₂₅H₁₆O₉
• CAS Number: 124387-19-5
• Molecular Weight: 460.39
• NACRES: NA.32
• PubChem Substance ID: 329752214
• UNSPSC Code: 12352108
• MDL number: MFCD00467494

Properties

• product line: BioReagent
• Quality Level: 100
• assay: ≥90.0% (HPLC)
• form: powder
• solubility: DMSO: soluble
• fluorescence: λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (esterase)
• suitability: suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1.CC(=O)Oc6ccc7c(Oc8cc(OC(C)=O)ccc8C79OC(=O)c%10cccc(C(O)=O)c9%10)c6
• InChI: 1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)
• InChI key: SWRGCNMDGRMQGB-UHFFFAOYSA-N

Description

General description

5(6)-Carboxyfluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe.[1] It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.[2]

Application

5(6)-Carboxyfluorescein diacetate (CFDA) is a dye which is been extensively utilized to label cultured neurons with the essential requirements, in a specific manner.[3]

5(6)- Carboxyfluorescein diacetate (CFDA) is a viability probe that assesses enzymatic activity, which is necessary to activate its fluorescence, and cell-membrane integrity, which is necessary for the retention of their fluorescent product within the cell.[4][5]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)