106-05A

Human Dermal Fibroblasts: HDF, adult

• Synonym(s): HDF cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human neonatal foreskin or adult skin (normal)
• Growth mode: Adherent
• Morphology: Fibroblast

Properties

• biological source: human neonatal foreskin or adult skin (normal)
• Quality Segment: 100
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Fibroblast
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

Application

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

Biochem/physiol Actions

Skin

Preparation Note

Fibroblast Growth Medium (116-500) all-in-one ready to use media

• Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
• Can be cultured at least 16 doublings.

Please refer to the HDF Culture Protocol.

Other Notes

Fibroblast Basal Medium containing 10% FBS & 10% DMSO.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable