11945

Benedict′s reagent

for the qualitative determination of sugars

• CAS Number: 63126-89-6
• UNSPSC Code: 12164500
• NACRES: NA.21
• MDL number: MFCD00146068

Properties

• Quality Level: 100
• technique(s): photometry: suitable
• impurities: cupric sulfate
• application(s): food and beverages; general analytical
• SMILES string: [Cu+2].[Na+].[Na+].[S](=O)(=O)(O)O.[O-]C(=O)C(O)(CC(=O)[O-])CC(=O)[O-].[O-]C(=O)O.O
• InChI: 1S/C6H8O7.CH2O3.Cu.2Na.H2O4S.H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;2-1(3)4;;;;1-5(2,3)4;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);(H2,2,3,4);;;;(H2,1,2,3,4);1H2/q;;+2;2*+1;;/p-4
• InChI key: QFKWSRIUZIYLCK-UHFFFAOYSA-J

Description

General description

Benedict′s reagents comprise of copper sulphate, sodium carbonate which functions as a mild alkali, and sodium citrate. It is quite stable. On boiling this reagent with glucose, it gives a white precipitate of cuprous thiocyanate. It is primarily used for the estimation of glucose in urine.

Application

Benedict′s reagent may be used during TLC analysis of mesocotyl methanol extracts during a study to evaluate sorghum resistance associated with accumulation of undetected toxic host metabolites.[1] It may also be also be used to synthesize an SEM image of the cubic shaped Cu₂O with smooth surface.[2]

Other Notes

Reagent used for the detection of reducing sugars and other reducing substances; detection limit: 0.01 % glucose in water

Safety Information

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H319,H411
• pcodes: P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P391
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves