This kit has been validated for use with stem cells. Osteoblasts are differentiated cells, and while hFOB1.19 may have alkaline phosphatase activity, the sensitivity to the detection method used in this kit cannot be predicted.
SCR004
Alkaline Phosphatase Detection Kit
This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.
Alkaline Phosphatase Detection Kit
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
ALP Stain, Alkaline Phosphtase Stem Cell Staining Kit, Stem Cell AP Staining Kit
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.84
eCl@ss:
32161000
Pricing and availability is not currently available.
Quality Level
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type cardiac stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
shipped in
wet ice
storage temp.
2-8°C
General description
Millipore′s Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
Application
Embryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore′s proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.
Millipore′s Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].
For Research Use Only; Not for use in diagnostic procedures
Millipore′s Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].
For Research Use Only; Not for use in diagnostic procedures
Research Category
Stem Cell Research
Stem Cell Research
Preparation Note
The Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.
When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
Other Notes
Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.
Napthol AS-BI phosphate solution (2mg/mL) in a buffer, pH 8.5 (Part No. CS235583). Two bottles.
Napthol AS-BI phosphate solution (2mg/mL) in a buffer, pH 8.5 (Part No. CS235583). Two bottles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | stem cell: suitable | technique(s) - | technique(s) microbe id | specific enzyme detection: suitable | technique(s) protein staining: suitable |
| species reactivity (predicted by homology) mammals | species reactivity (predicted by homology) - | species reactivity (predicted by homology) - | species reactivity (predicted by homology) - |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. - |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| input sample type cardiac stem cell(s) | input - | input - | input - |
signalword
Warning
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
hcodes
Hazard Classifications
Carc. 2
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCR004 | 04053252503757 |
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Can I use this kit for the determination of ALP activity in hFOB1.19 Osteoblasts?
1 answer-
Helpful?
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Can I use it for mesenchimal stem cells
1 answer-
Yes, as indicated in the 'PROPERTIES' section, this kit may be used for the determination of Alkaline Phosphatase activity in mesenchymal stem cells.
Helpful?
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