MAB4360

Anti-TRA-1-60 Antibody, clone TRA-1-60

clone TRA-1-60, Chemicon^®, from mouse

• Synonym(s): TRA-1-60 Marker
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: TRA-1-60, monoclonal
• Species reactivity: human
• Application: FACS, ICC, IF, IP, WB
• Citations: 391

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: TRA-1-60, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• input: sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• isotype: IgM
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PODXL(5420)

Description

General description

235/410 kDa

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Application

Immunofluorescence: A previous lot was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60.

Biochem/physiol Actions

This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.

Physical form

Format: Purified

Purified mouse monoclonal IgM liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.

Preparation Note

Stable for 1 year at from date of receipt.

Analysis Note

Control
NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg