MAB1334

Anti-Collagen Type I, II & III Antibody

CHEMICON^®, mouse monoclonal, MMCHABC

• Synonym(s): Interstitial Collagens
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: MMCHABC, monoclonal
• Species reactivity: human
• Application: ELISA, IHC, RIA
• Citations: 2

Properties

• Product Name: Anti-Collagen Types I, II & III Antibody, clone MMCHABC, clone MMCHABC, Chemicon^®, from mouse
• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MMCHABC, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable
• isotype: IgM
• suitability: not suitable for Western blot
• NCBI accession no.: NM_000088.3
• UniProt accession no.: P02452, P02458, P02461
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... COL1A1(1277)

Description

Immunogen

Human collagen type I

Application

Anti-Collagen Types I, II & III Antibody, clone MMCHABC detects level of Collagen Types I & has been published & validated for use in ELISA, RIA, IH.

ELISA: Apparent binding constant from titration curves is 0.1 nM. Can be used as capture or reporter antibody for determination of collagen I or III in sandwich ELISA, but requires combination with collagen type-specific antibody for other function.

Immunohistochemistry: cryostat sections fixed with acetone or ethanol. Staining pattern similar to polyclonal antibody to interstitial collagens. Formaldehyde fixation is not recommended.

Not suitable for use on paraffin embedded sections or immunoblots.

Optimal working dilution must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Biochem/physiol Actions

Human collagen types I, II and III (both native and denatured). No cross reactivity with human plasma proteins, other collagen types or non-collagenous proteins in ELISA, RIA or immunoblot.

Physical form

Format: Purified

Purified from murine ascites by PEG precipitation and Sepharose^™ CL-4B chromatography. Lyophilized Powder.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sepharose is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H360FD
• pcodes: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects