71456
BugBuster® Master Mix
Synonym(s):
BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme solution, Cell Lysis Master Mix
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About This Item
NACRES:
NA.32
UNSPSC Code:
41106511
Pricing and availability is not currently available.
form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
2-8°C
General description
The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.
Application
BugBuster® Master Mix has been used:
- to resuspend and lyse the cell pellets during protein purification
- to extract proteins for immunoblotting
- as a bacterial lysis buffer for the preparation of inclusion bodies
Biochem/physiol Actions
BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.
Features and Benefits
- Efficient lysis of Gram-positive and Gram-negative bacteria
- Maximum recovery of active, soluble protein
- Capable of cell wall perforation without denaturing soluble protein
- Useful for the preparation of high purity inclusion bodies
- Compatible with protease and phosphatase inhibitor cocktail
- Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
- The Master Mix eliminates the need to dilute or perform separate addition steps.
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| shipped in wet ice | shipped in ambient | shipped in wet ice | shipped in wet ice |
| storage temp. 2-8°C | storage temp. 10-30°C | storage temp. 2-8°C | storage temp. - |
| form liquid | form liquid | form liquid | form liquid |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level - |
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Matthew Thomas Doyle et al.
Molecular cell, 81(9), 2000-2012 (2021-03-12)
The β-barrel assembly machine (BAM) integrates β-barrel proteins into the outer membrane (OM) of Gram-negative bacteria. An essential BAM subunit (BamA) catalyzes integration by promoting the formation of a hybrid-barrel intermediate state between its own β-barrel domain and that of
Development of a synthetic biosensor for chemical exchange MRI utilizing in silico optimized peptides
Fillion AJ, et al.
Nmr in Biomedicine, 36, e5007-e5007 (2023)
FMRP inhibits translation elongation independent of mRNA G-quadruplexes
Scarpitti MR, et al.
bioRxiv : the preprint server for biology null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 71456-3 | 07790788053260 |
| 71456-4 | 04055977270150 |