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Magna ChIP® Protein G Magnetic Beads

Recombinant Protein G covalently bound to magnetic beads for use in chromatin immunoprecipitations (ChIP assays). These protein G beads provide users a more rapid, reproducible & efficient reagent for collecting immunocomplexes vs. agarose beads.

Magna ChIP® Protein G Magnetic Beads

Synonym(s):

ChIP magnetic beads, ChIP magnetic G beads, Magnetic Chromatin Immunoprecipitation

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About This Item

UNSPSC Code:
41105507
NACRES:
NA.52
eCl@ss:
32160405
Pricing and availability is not currently available.
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Quality Segment

packaging

pkg of 1 mL

manufacturer/tradename

Magna ChIP®

particle size

~3 μm

shipped in

wet ice

storage temp.

2-8°C

General description

Recombinant protein G covalently bound to magnetic beads. These beads provide users a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitations (ChIP) assays. Compared with conventional protein G agarose beads, protein G magnetic beads significantly reduce the handling time and mechanical stress on target immunocomplexes.

Application

Recombinant Protein G covalently bound to magnetic beads for use in chromatin immunoprecipitations (ChIP assays). These protein G beads provide users a more rapid, reproducible & efficient reagent for collecting immunocomplexes vs. agarose beads.
Research Category
Epigenetics & Nuclear Function
Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.
Used to detect/quantify: Protein G

Physical form

Liquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of shipment. Do Not Freeze.

Analysis Note

Routinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP® A Kit (Cat. #17-610).

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
16-66116-66317-10085
storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

packaging

pkg of 1 mL

packaging

pkg of 1 mL

packaging

pkg of 1 mL

packaging

-

particle size

~3 μm

particle size

~3 μm

particle size

~3 μm

particle size

-

manufacturer/tradename

Magna ChIP®

manufacturer/tradename

Magna ChIP®

manufacturer/tradename

Magna ChIP®

manufacturer/tradename

Magna ChIP®


Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

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Related Content

Chromatin-immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-seq) of the immunoprecipitated DNA is a powerful tool for the investigation of protein:DNA interactions. To perform ChIP-seq, chromatin is isolated from cells or tissues (with or without chemical crosslinking) and fragmented. Antibodies recognizing chromatinassociated proteins of interest are used to enrich the sample for specific chromatin fragments. The DNA is recovered, sequenced on various NGS platforms, and aligned to a reference genome to determine specific protein binding loci. ChIP-seq studies have increased our knowledge of transcription factor biology, DNA methylation and histone modifications.

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

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Global Trade Item Number

SKUGTIN
16-66204053252377167
16-662X04053252377174

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