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Anti-NET5 Antibody

Name: Anti-NET5 Antibody
Brand: MM

from rabbit, purified by affinity chromatography

Synonym(s):

transmembrane protein 201, Spindle-associated membrane protein 1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ICC, WB

Citations:

Pricing and availability is not currently available.

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

species reactivity (predicted by homology)

orangutan

technique(s)

immunocytochemistry: suitable, western blot: suitable

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This Item	MABE1807	C7488	MABE866
Sigma-Aldrich 06-1013 Anti-NET5 Antibody	Sigma-Aldrich MABE1807 Anti-NuMA1 Antibody, clone AD6-1	$Anti-CENP-E antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C7488 Anti-CENP-E antibody produced in rabbit	Sigma-Aldrich MABE866 Anti-Mad2 Antibody, clone AS55-A12
clone polyclonal	clone AD6-1, monoclonal	clone polyclonal	clone AS55-A12, monoclonal
biological source rabbit	biological source mouse	biological source rabbit	biological source mouse
antibody form affinity isolated antibody	antibody form purified antibody	antibody form IgG fraction of antiserum	antibody form purified immunoglobulin
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity rat, human, mouse	species reactivity human, mouse	species reactivity human	species reactivity human
Gene Information human ... TMEM201(199953)	Gene Information human ... NUMA1(4926)	Gene Information human ... CENPE(1062)	Gene Information human ... MAD2L1(4085)

General description

Transmembrane protein 201, also known as NET5, is a transmembrane protein of the nuclear envelope that is homologous to spindle-associated membrane protein 1 (SAMP1) in humans. NET5 is thought to be expressed on the inner nuclear membrane during interphase and move to the polar regions of the mitotic spindle during mitosis.

~72 and/or 43 kDa observed depending on lysate used. Isoform1: 72 kDa and Isoform 2: 43 kDa Uncharacterized band at ~28 kDa may be observed in some lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human NET5.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-NET5 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect NET5 also known as transmembrane protein 201.

Western Blot Analysis: 1 µg/mL from a representative lot detected NET5 on 10 µg of rat heart tissue lysate.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory that detected NET5 in U2OS cells expressing various NET-GFP fusions. (Wilkie, G., et al. (2011) Mol. Cell Proteomics).

Physical form

Affinity purified

Purified rabbit polyclonal containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human heart tissue lysate

Evaluated by Western Blot in human heart tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected NET5 on 10 µg of human heart tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lamin A/C Assembly Defects in LMNA-Congenital Muscular Dystrophy Is Responsible for the Increased Severity of the Disease Compared with Emery-Dreifuss Muscular Dystrophy.

Anne T Bertrand et al.

Cells, 9(4) (2020-04-05)

LMNA encodes for Lamin A/C, type V intermediate filaments that polymerize under the inner nuclear membrane to form the nuclear lamina. A small fraction of Lamin A/C, less polymerized, is also found in the nucleoplasm. Lamin A/C functions include roles

Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery.

Nikolaj Zuleger et al.

Genome biology, 14(2), R14-R14 (2013-02-19)

Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells

Immunohistochemistry on a panel of Emery-Dreifuss muscular dystrophy samples reveals nuclear envelope proteins as inconsistent markers for pathology.

Phu Le Thanh et al.

Neuromuscular disorders : NMD, 27(4), 338-351 (2017-02-19)

Reports of aberrant distribution for some nuclear envelope proteins in cells expressing a few Emery-Dreifuss muscular dystrophy mutations raised the possibility that such protein redistribution could underlie pathology and/or be diagnostic. However, this disorder is linked to 8 different genes

The nuclear envelope proteome differs notably between tissues.

Nadia Korfali et al.

Nucleus (Austin, Tex.), 3(6), 552-564 (2012-09-20)

One hypothesis to explain how mutations in the same nuclear envelope proteins yield pathologies focused in distinct tissues is that as yet unidentified tissue-specific partners mediate the disease pathologies. The nuclear envelope proteome was recently determined from leukocytes and muscle.

Several novel nuclear envelope transmembrane proteins identified in skeletal muscle have cytoskeletal associations.

Gavin S Wilkie et al.

Molecular & cellular proteomics : MCP, 10(1), M110-M110 (2010-09-30)

Nuclear envelopes from liver and a neuroblastoma cell line have previously been analyzed by proteomics; however, most diseases associated with the nuclear envelope affect muscle. To determine whether muscle has unique nuclear envelope proteins, rat skeletal muscle nuclear envelopes were

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