T9701

TRAIL human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE and HPLC), lyophilized powder

• Synonym(s): Apo2 Ligand, Apo2L, TL2, TNF-related apoptosis-inducing ligand
• CAS Number: 2244730-93-4
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD01867158
• Form: lyophilized powder
• Assay: ≥98% (SDS-PAGE and HPLC)
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: 19.6 kDa, predicted mol wt ~21 kDa

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• description: extracellular domain
• assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• mol wt: 19.6 kDa, predicted mol wt ~21 kDa
• technique(s): immunofluorescence: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P50591
• storage temp.: −20°C
• Gene Information: human ... TNFSF10(8743)

Description

Application

TRAIL human has been used in immunofluorescence analyses, to study the delocalization of melanoma antigen-D2 in the nucleoplasm after a cellular stress.

Human TRAIL can be used as a selection agent for treating human cells for phenotypic enrichments.[1] The product can also be used in chemical transfection for identifying Small RNA Modulators of TRAIL-Induced Apoptosis Pathway by utilizing libraries of synthetic small interfering RNA (siRNA) and microRNAs (miRNA).[2]

Biochem/physiol Actions

TRAIL is a cytokine with anti-inflammatory, and antitumor activity. It is expressed in various cells including immune cells, vascular cells and adipocytes. TRAIL is associated with vascular endothelial cell protection.

TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. The product present in both soluble as well as membrane-bound form, can induce rapid apoptosis of various transformed cell lines but not the normal cells.[3] Additionally, the product can also induce apoptotic cell death in TRAIL-sensitive U343MG cells.

Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.

Physical form

Lyophilized from phosphate buffered saline

Analysis Note

The biological activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive LN-18 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable