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M0769

Minimum Essential Medium Eagle

AutoMod™, with Earle′s salts, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

MEM

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About This Item

NACRES:
NA.75
UNSPSC Code:
12171500
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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
phenol red: yes
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

Modification of the original MEM formulation. Contains a succinate buffering system. See formulations table for specific information. Medium may be autoclaved.

Preparation Note

Formulated to contain 9.4 grams of powder per liter of medium.
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Other Notes

Modified for autoclaving.

Legal Information

Auto-Mod is a trademark of Sigma-Aldrich Co. LLC

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M0769

Minimum Essential Medium Eagle

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Minimum Essential Medium Eagle

Minimum Essential Medium Eagle Spinner Modification, with Earle′s salts and sodium bicarbonate, without calcium chloride and L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Minimum Essential Medium Eagle

Minimum Essential Medium Eagle Joklik Modification, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M8028

Minimum Essential Medium Eagle

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

liquid

form

liquid

components

NaHCO3: no
phenol red: yes
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
HEPES: no

components

HEPES: no
sodium pyruvate: no
L-glutamine: yes
NaHCO3: no
phenol red: yes

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: yes
L-glutamine: no
HEPES: no

components

glucose: yes
phenol red: yes
HEPES: no
Hanks’ salts (2% CO2): no
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): no
sodium pyruvate: no
stable glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

400

Quality Level

400

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000449617
0000449617
0000446765
0000446765
0000443886

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Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Hisashi Miura et al.
Nucleic acids research, 37(3), 778-792 (2008-12-17)
Mammalian tissue- and/or time-specific transcription is primarily regulated in a combinatorial fashion through interactions between a specific set of transcriptional regulatory factors (TRFs) and their cognate cis-regulatory elements located in the regulatory regions. In exploring the DNA regions and TRFs
Barbara Zonta et al.
Neuron, 69(5), 945-956 (2011-03-09)
The axon initial segment (AIS) is critical for the initiation and propagation of action potentials. Assembly of the AIS requires interactions between scaffolding molecules and voltage-gated sodium channels, but the molecular mechanisms that stabilize the AIS are poorly understood. The
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Global Trade Item Number

SKUGTIN
M0769-500L04065265666398
M0769-50L04061837459566
M0769-100L04065265666374
M0769-BIBC04061826254165
M0769-250L04065265666381
M0769-10X1L04061834037415

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