The exact number of cells may vary depending on cell type and cell condition. The recommended concentration to use for labeling is 10-100ug/ml (~7.4 mM-74mM), which is mixed in a 1:1 volume with cells.
F1058
FSL-Fluorescein
Synonym(s):
FSL-FLRO4(fluorescein)-SA2-L1
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About This Item
Empirical Formula (Hill Notation):
C73H109N4O15PS
Molecular Weight:
1345.70
UNSPSC Code:
41116100
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.
Quality Segment
storage temp.
−20°C
SMILES string
CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(=O)OCCNC(=O)CCCCC(=O)NCCCCCNC(=S)Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35)OC(=O)CCCCCCC\C=C/CCCCCCCC
InChI
1S/C73H109N4O15PS/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-32-40-69(82)87-55-60(90-70(83)41-33-30-28-26-24-22-20-18-16-14-12-10-8-6-4-2)56-89-93(85,86)88-51-50-75-68(81)39-35-34-38-67(80)74-48-36-31-37-49-76-72(94)77-57-42-45-62-61(52-57)71(84)92-73(62)63-46-43-58(78)53-65(63)91-66-54-59(79)44-47-64(66)73/h17-20,42-47,52-54,60,78-79H,3-16,21-41,48-51,55-56H2,1-2H3,(H,74,80)(H,75,81)(H,85,86)(H2,76,77,94)/b19-17-,20-18-
InChI key
OZXSVOLFZKVFHP-CLFAGFIQSA-N
Application
All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with FSL constructs are known as kodecytes and usually maintain their normal vitality and functionality. FSL-fluorescein has been specifically designed to create fluorescein-labeled live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. Fluorescein-labeled kodecytes can be visualized at the approximate absorbance wavelength of 488 nm and emission at 518 nm.
All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. FSL-fluorescein has been specifically designed to create fluorescein-labeled live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces.
Other Notes
FSL-fluorescein is a KODE™ technology construct designed to label hydrophobic surfaces including living cells with biotin. All FSL constructs consist of three essential designable features:
- functional component (F)
- spacer (S)
- diacyl lipid (L)
Packaged as 1 mg FSL-Fluorescein with 0.42 mg sodium bicarbonate to improve solubility.
Legal Information
KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.
KODE is a trademark of KODE Biotech Ltd.
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| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How many cells can I label with product F1058, FSL-Fluorescein?
1 answer-
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May I use serum with the product F1058, FSL-Fluorescein?
1 answer-
We do not recommend diluting the product F1058, FSL-Fluorescein, in lipid-containing buffers. The FSL will associate with the lipids and reduce the efficiency of insertion into cell membranes.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What should I use to reconstitute product F1058, FSL-Fluorescein?
1 answer-
We recommend reconstituting 1 mg of product F1058, FSL-Fluorescein with 0.5ml of saline or PBS for a concentration of 2 mg/ml.
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Will the product F1058, FSL-Fluorescein label dead cells?
1 answer-
The product F1058, FSL-Fluorescein, is designed to insert into the membranes of live cells. But it can also be used to label fixed cells, enveloped virions, and solid phase surfaces.
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What concentration of F1058, FSL-Fluorescein do I use to label cells?
1 answer-
For cell labeling we recommend using a concentration of 10-100ug/ml of F1058, FSL-Fluorescein diluted in PBS. Label cells with FSL-Fluorescein in PBS at a 1:1 volume to volume ratio. The exact concentration may need to be optimized for the cells and the application. Keep incubations in the dark.
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