75259

Tris(2-carboxyethyl)phosphine hydrochloride

BioUltra, ≥98% (NMR)

• Synonym(s): TCEP
• Empirical Formula (Hill Notation): C₉H₁₅O₆P · HCl
• CAS Number: 51805-45-9
• Molecular Weight: 286.65
• UNSPSC Code: 12352128
• NACRES: NA.25
• PubChem Substance ID: 329766294
• MDL number: MFCD00145469
• Beilstein/REAXYS Number: 3724376

Properties

• product line: BioUltra
• Quality Segment: 300
• assay: ≥98% (NMR)
• form: crystals
• reaction suitability: reagent type: reductant
• loss: ≤1.0% loss on drying
• pH: 1.2-1.5
• solubility: H₂O: 1 M, clear, colorless (no residue)
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.05 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.05, λ: 280 nm A_max: 0.03
• storage temp.: 2-8°C
• SMILES string: Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O
• InChI: 1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
• InChI key: PBVAJRFEEOIAGW-UHFFFAOYSA-N

Description

General description

Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution.[1][2] It readily and stoichiometrically reduces disulfides with high specificity.[1] TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.[2]

Application

Tris(2-carboxyethyl)phosphine hydrochloride has been used:

• as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA[3]
• in reducing sulfhydryl groups to obtain total thiol fraction[4]
• to reduce protein samples[5]

Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H314
• pcodes: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Storage Class: 8A - Combustible corrosive hazardous materials
• wgk: WGK 1