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7-(Diethylamino)coumarin-3-carbohydrazide

Name: 7-(Diethylamino)coumarin-3-carbohydrazide
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥95% (HPCE)

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₇N₃O₃

CAS Number:

100343-98-4

Molecular Weight:

275.30

UNSPSC Code:

12352125

NACRES:

NA.32

PubChem Substance ID:

57649662

MDL number:

MFCD00467583

Beilstein/REAXYS Number:

5574454

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Properties

product line

BioReagent

Quality Level

100

assay

≥95% (HPCE)

solubility

DMF: soluble, acetonitrile: soluble, chloroform: soluble, methanol: soluble

fluorescence

λ_ex 450 nm; λ_em 468 nm in methanol

suitability

suitable for fluorescence

SMILES string

CCN(CC)c1ccc2C=C(C(=O)NN)C(=O)Oc2c1

InChI

1S/C14H17N3O3/c1-3-17(4-2)10-6-5-9-7-11(13(18)16-15)14(19)20-12(9)8-10/h5-8H,3-4,15H2,1-2H3,(H,16,18)

InChI key

LYBMHAINSFEHRL-UHFFFAOYSA-N

Description

General description

7-(Diethylamino)coumarin-3-carbohydrazide is a coumarin-hydrazide reagent typically used as a fluorescent chemical probe for labeling of cellular carbonyls.

Application

7-(Diethylamino)coumarin-3-carbohydrazide is used for derivatization and detection of carboxylic acid and carbonylated molecules such as lipid peroxidation products (LPP) by electrospray ionization-mass spectrometry (ESI-MS) in positive ion mode. It may be used as a fluorescent label for the determination of lipid aldehydes in food products by normal phase liquid chromatography (NPLC) coupled to ESI-MS and fluorescent detector (FLD) and for the derivatization of monoterpene alcohols in urine prior to analysis by high performance liquid chromatography(HPLC) with FLD coupled to ESI-MS.[1]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivatizing agent for carboxylic acid detection

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This Item	442631	C1484	248924
Sigma-Aldrich 36798 7-(Diethylamino)coumarin-3-carbohydrazide	Sigma-Aldrich 442631 Coumarin 6	Sigma-Aldrich C1484 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin	Sigma-Aldrich 248924 7-Amino-4-(trifluoromethyl)coumarin
suitability suitable for fluorescence	suitability -	suitability -	suitability -
fluorescence λ_ex 450 nm; λ_em 468 nm in methanol	fluorescence λ_em 505 nm in ethanol (Lasing peak 534 nm, lasing range 515 - 558 nm (DMSO), pump source XeCl (308 nm))	fluorescence -	fluorescence -
solubility DMF: soluble, chloroform: soluble, acetonitrile: soluble, methanol: soluble	solubility -	solubility DMSO: soluble, methanol: soluble	solubility -
assay ≥95% (HPCE)	assay 98%	assay ≥95% (HPLC)	assay ≥99%
product line BioReagent	product line -	product line -	product line -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Simultaneous detection of low and high molecular weight carbonylated compounds derived from lipid peroxidation by electrospray ionization-tandem mass spectrometry.

Ivana Milic et al.

Analytical chemistry, 85(1), 156-162 (2012-11-29)

Reactive oxygen species (ROS) and other oxidative agents such as free radicals can oxidize polyunsaturated fatty acids (PUFA) as well as PUFA in lipids. The oxidation products can undergo consecutive reactions including oxidative cleavages to yield a chemically diverse group

Sensitive determination of monoterpene alcohols in urine by HPLC-FLD combined with ESI-MS detection after online-solid phase extraction of the monoterpene-coumarincarbamate derivates.

Roland J W Meesters et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 875(2), 444-450 (2008-10-11)

A method was developed for the determination of the monoterpene alcohols verbenol, myrtenol, perillyl alcohol, alpha-terpineol, Delta(3)-carene-10-ol, thymol and p-alpha,alpha-trimethylbenzylalcohol in urine samples. After an enzymatic cleavage of their glucuronide- and sulfate conjugates the monoterpene alcohols were converted in the

M.L. Grayeski et al.

Analytical Chemistry, 59, 1203-1203 (1987)

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Global Trade Item Number

SKU	GTIN
36798-25MG-F	04061832531694

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