According to ECACC, D6546 (DMEM) and G7513 (glutamine) are on the recommended product list for reagents required in cell culture applications.
D6546
DMEM - high glucose
With sodium Bicarbonate, sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
1 of 2 reviewers received a sample product or took part in a promotion
Synonym(s):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
Product Name
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high
shipped in
ambient
storage temp.
2-8°C
General description
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Preparation Note
Supplement with 0.584 g/L L-glutamine.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Gloria Bonuccelli et al.
Oncotarget, 8(13), 20667-20678 (2017-02-23)
Here, we assembled a broad molecular "tool-kit" to interrogate the role of metabolic heterogeneity in the propagation of cancer stem-like cells (CSCs). First, we subjected MCF7 cells to "metabolic fractionation" by flow cytometry, using fluorescent mitochondrial probes to detect PCG1α
Morag R Hunter et al.
Journal of molecular biology, 430(14), 2153-2163 (2018-05-21)
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Maria Peiris-Pagès et al.
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Using proteomics analysis, we previously compared MCF7 breast cancer cells grown as 3D tumor spheres, with the same cell line grown as monolayers. Our results indicated that during 3D anchorage-independent growth, the cellular machinery associated with i) mitochondrial biogenesis and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D6546-24X500ML | 04061833587768 |
| D6546-6X500ML | 04061833587782 |
| D6546-500ML | 04061833587775 |
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Which DMEM media and glutamine products would you recommend for the PEA-13-97040311 cell line purchased from ECACC?
1 answer-
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