C270

Monoclonal Anti-Calpastatin antibody produced in mouse

clone 1F7E3D10, ascites fluid, buffered aqueous solution

• Synonym(s): Anti-BS-17, Anti-PLACK
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164555
• Conjugate: unconjugated
• Clone: 1F7E3D10, monoclonal
• Application: IF, WB
• Citations: 10

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 1F7E3D10, monoclonal
• form: buffered aqueous solution
• species reactivity: human, rat, bovine, pig
• technique(s): indirect immunofluorescence: 1:200, western blot: 1:5,000
• isotype: IgG1
• UniProt accession no.: P20810
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CAST(831) ; rat ... Cast(25403)

Description

General description

Calpastatin is a protein encoded by the CAST gene in humans. Calpastatin is an endogenous inhibitor of the ubiquitous calcium dependent proteinases μ- and m-calpain. It plays a key role in many cellular physiological and pathological processes. Calpastatin level is very important for controlling calpain activity.

Immunogen

calpastatin from bovine skeletal muscle.

Application

Monoclonal Anti-Calpastatin antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:200 and for western blotting at a dilution of 1:5,000.

Biochem/physiol Actions

Calpastatin availability in cytosol is controlled by Ca²⁺ and cyclic AMP. Prolonged calpain activation promotes degradation of calpastatin. Calpastatin is found to be overexpressed in mycoplasma-contaminated cultured cells. CAST gene is found to be involved in genetic susceptibility to Keratoconus (KC), a genetically heterogeneous corneal dystrophy. It is also found to be associated with Parkinson′s disease (PD) and single nucleotide polymorphisms (SNPs).

Epitope mapping studies indicate the epitope is between amino acids 543-673 (domain IV) of human calpastatin. By immunoblotting, the antibody may detect bands at 150, 125, 90 and 70 kDa, which have been suggested to be proteins translated from different start sites of the calpastatin gene. It does not cross-react with calpains or calmodulin. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and in rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.

Physical form

Solution in phosphate buffered saline and contains 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)