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DMEM/F-12

Name: DMEM/F-12
Brand: SIGMA

With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure

Synonym(s):

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

Pricing and availability is not currently available.

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Properties

Product Name

DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl_2,MgCl₂, MgSO_4,sodium bicarbonate, and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO₃: no
phenol red: no
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

Description

General description

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.

Preparation Note

Formulated to contain 14.8 grams of powder per liter of medium.

Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

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This Item	D8900	D8062	D8437
SAFC D9785 DMEM/Nutrient Mixture F-12 Ham	Sigma-Aldrich D8900 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D8062 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D8437 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form powder	form liquid	form liquid
components L-glutamine: no HEPES: 15 mM sodium pyruvate: 0.055 g/L NaHCO₃: no phenol red: no glucose: 3.15 g/L	components sodium pyruvate: 0.055 g/L HEPES: 15 mM NaHCO₃: no L-glutamine: yes glucose: 3.15 g/L phenol red: yes	components glucose: 3.15 g/L NaHCO₃: yes L-glutamine: yes phenol red: yes HEPES: no sodium pyruvate: 0.055 g/L	components glucose: 3.15 g/L HEPES: yes L-glutamine: yes phenol red: yes sodium pyruvate: 0.055 g/L
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
DME/Ham′s F-12, FBS, low-serum	DME/Ham′s F-12, FBS, low-serum	DME/Ham′s F-12, FBS, low-serum	classic media, classical media, serum supplement, FBS, high glucose

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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