D9780

Lambda DNA Hind III Digest

for DNA electrophoresis

• Synonym(s): Hind III digested bacteria phage Lambda DNA
• NACRES: NA.25
• UNSPSC Code: 41141827
• Form: liquid
• Storage temp.: −20°C

Properties

• grade: Molecular Biology
• Quality Level: 200
• form: liquid
• usage: 100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)
• suitability: suitable for electrophoresis (DNA)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Other Notes

Contains 8 fragments, 125–23,130 bp

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable