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1414003

USP

Methotrexate

United States Pharmacopeia (USP) Reference Standard

Synonym(s):

(+)-Amethopterin, 4-Amino-10-methylfolic acid, 4-Amino-N10-methylpteroyl-L-glutamic acid, Antifolan, MTX, Methylaminopterin

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About This Item

Empirical Formula (Hill Notation):
C20H22N8O5
CAS Number:
59-05-2
Molecular Weight:
454.44
UNSPSC Code:
41116107
NACRES:
NA.24
PubChem Substance ID:
329750410
MDL number:
MFCD00064370
Beilstein/REAXYS Number:
70669
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grade

pharmaceutical primary standard

API family

methotrexate

manufacturer/tradename

USP

application(s)

pharmaceutical (small molecule)

format

neat

storage temp.

2-8°C

SMILES string

CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O

InChI

1S/C20H22N8O5/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27)/t13-/m0/s1

InChI key

FBOZXECLQNJBKD-ZDUSSCGKSA-N

Gene Information

human ... DHFR(1719)

General description

This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product, including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia.For further information and support please go to the website of the issuing Pharmacopoeia.

Application

Methotrexate USP Reference standard, intended for use in specified quality tests and assays as specified in the USP compendia. Also, for use with USP monographs such as:
  • Methotrexate Injection
  • Methotrexate Tablets
  • Methotrexate for Injection

Analysis Note

These products are for test and assay use only. They are not meant for administration to humans or animals and cannot be used to diagnose, treat, or cure diseases of any kind.  ​

Other Notes

Sales restrictions may apply.

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Methotrexate United States Pharmacopeia (USP) Reference Standard

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1414003

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Methotrexate European Pharmacopoeia (EP) Reference Standard

M1000000

Methotrexate

Methotrexate Pharmaceutical Secondary Standard; Certified Reference Material

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grade

pharmaceutical primary standard

grade

pharmaceutical primary standard

grade

certified reference material, pharmaceutical secondary standard

grade

pharmaceutical primary standard

manufacturer/tradename

USP

manufacturer/tradename

EDQM

manufacturer/tradename

-

manufacturer/tradename

EDQM

format

neat

format

neat

format

neat

format

neat

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

Gene Information

human ... DHFR(1719)

Gene Information

human ... DHFR(1719)

Gene Information

human ... DHFR(1719)

Gene Information

human ... DHFR(1719)

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

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pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Muta. 2 - Repr. 1B - STOT RE 1

target_organs

Liver,Bone marrow

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKUGTIN
1414003-500MG04061838734051

Questions

  1. hello, Can I use methotrexate 1414003 in cell culture? If yes, what should I use to disolve?

    1 answer

    1. Product number 1414003 is a United States Pharmacopeia (USP) reference standard used in analytical method validation, system suitability testing, assays, and quality control checks. It has not been validated for use in cell culture applications.

      Product number M8407 is a methotrexate hydrate that has been tested for cell culture: https://www.sigmaaldrich.com/US/en/product/sigma/m8407

      Methotrexate is insoluble in water, ethanol, chloroform, and ether. It is, however, soluble in solutions of mineral acids and in dilute solutions of alkali hydroxides and carbonates. (1) Methotrexate is unstable in basic solution. (2) For cell culture work, a stock solution of methotrexate is prepared using a minimum amount of 1 M NaOH and then diluted with saline or medium. The diluted stock remains active at 4–8 °C for about a week or at –20 °C for about a month.

      References
      1. Martindale: the Extra Pharmacopoeia, 29th ed., p. 636 (1989).
      2. Merck Index, 12th ed., #6065 (1996).

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