T9253

Trypsin inhibitor

powder

• Synonym(s): Ovomucoid
• CAS Number: 9035-81-8
• UNSPSC Code: 12352202
• NACRES: NA.77
• EC Number: 232-906-9
• MDL number: MFCD00082102

Properties

• Product Name: Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor
• biological source: chicken egg white
• Quality Segment: 200
• type: Type II-O
• form: powder
• mol wt: 28 kDa
• solubility: water: soluble 10 g/L
• storage temp.: 2-8°C

Description

General description

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

25% of ovomucoid comprises carbohydrate.

Application

Trypsin inhibitor from chicken egg white has been used:

• to produce denatured, oxidized and deglycosylated ovomucoid and used to stimulate patient derived cell cultures for mapping T cell epitopes
• to treat nuclei for flow cytometric analysis
• in Holtfreter′s solution, used for the dissociation of planarians into cells

Analysis Note

One mg will inhibit 0.8-1.6 mg of tryspin with activity of approx. 10,000 BAEE units per mg protein. May inhibit ≤0.3 mg of chymotrypsin with activity of approx. 40 BTEE units per mg protein.

Other Notes

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Ovomucoid, from chicken egg white, does not itself inhibit chymotrypsin. Chymotrypsin inhibition is a measure of ovoinhibitor contamination by method of Feeney, R. E., et al., J. Biol. Chem., 238, 1415 (1963).

View more information on Trypsin Inhibitor.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)