This powder is not sterile. Prepared solutions may be filter-sterilized. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/699/t0665dat.pdf
T0665
holo-Transferrin human
powder, BioReagent, suitable for cell culture, ≥97%
Synonym(s):
Siderophilin, iron-saturated
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About This Item
CAS Number:
UNSPSC Code:
12352202
EC Number:
234-318-8
NACRES:
NA.75
MDL number:
eCl@ss:
34058011
Pricing and availability is not currently available.
biological source
human plasma
Quality Level
description
Iron-saturated
product line
BioReagent
assay
≥97%
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods), endotoxin, tested
solubility
H2O: 50 mg/mL
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... TF(7018)
Application
holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.
Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.
Biochem/physiol Actions
Provides iron to cells in a physiologically stable and safe form.
Preparation Note
Heat treated at 60 °C (minimum) for a minimum of 10 hours.
Analysis Note
Iron content by ICP reported on a lot-specific basis.
Purity by agarose gel electrophoresis.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) immunoprecipitation (IP): suitable, tissue culture: suitable |
| form powder | form powder | form powder | form powder |
| biological source human plasma | biological source - | biological source human | biological source human |
| solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| T0665-100MG | 04061837335662 |
| T0665-1G | 04061837335679 |
| T0665-50MG | 04061837335723 |
| T0665-500MG | 04061837335709 |
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Is the powder sterile?
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What is the recommended storage method for stock solutions of item T0665, holo-Transferrin human?
1 answer-
Stock solutions remain stable for a period of up to two weeks when stored at 4°C.
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What's the process of saturation? Do the cells express directly holo-Tf with iron? Or apo-Tf reacts with iron salts?
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The delivery of iron to cells by transferrins is a receptor-mediated and controlled process. Cells regulate the amount of iron they receive from the extracellular environment by varying transferrin receptor expression. Two iron-loaded transferrin molecules bind to one transferrin receptor. Once bound, they are transferred into acidic endosomes within the cell’s interior. At reduced pH, the iron is released and incorporated into intracellular proteins and the storage molecule ferritin. The apo-transferrin (iron-free) is recycled back to the exterior of the cell and released. Each transferrin receptor cycle can deliver up to four atoms of iron.
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Holo-Tf is described as "iron saturated", but there is a statement "Under normal circumstances, approximately one-third of transferrin iron-binding pockets are filled." I'm confused if the product I got (T0665) is 100% saturated with iron or not.
1 answer-
This product is a Holo-Transferrin form which is generally considered to be iron-saturated. The specification for iron content of this product is 0.10 - 0.35%. Conversely, Apo-Transferrin is considered to be iron deficient. Apo-transferrin product T5391 has an iron content specification of <0.005%. Each Transferrin has the capacity to bind 2 iron (Fe3+) molecules. In a circulating system, the iron content is transitory and rarely (if ever) 100% saturated. This product is tested for total iron content. The number of binding sites that are filled is not measured. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/272/520/t0665pis.pdfHelpful?
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What is the recommended holo-transferrin solvent and how should the dissolved protein be stored?
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This product is tested for solubility in water at 50 mg/mL. Rates are expected to be comparable when dissolving in an aqueous buffer or medium. Solutions are stable for 5-10 days at 2-8°C. This product has not been evaluated for long-term solution stability. However, medium supplements containing transferrin are stable when stored in aliquots at -20°C for up to one year. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/699/t0665dat.pdfHelpful?
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What is the carbohydrate content of human transferrin?
1 answer-
We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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