SHP002
CRISPR & MISSION® Lentiviral Packaging Mix
Synonym(s):
Lentiviral Packaging Mix
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About This Item
NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
100 ng/μL (in Tris-EDTA)
Pricing and availability is not currently available.
concentration
100 ng/μL (in Tris-EDTA)
Quality Level
application(s)
CRISPR
shipped in
dry ice
storage temp.
−20°C
General description
The CRISPR & MISSION Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.
Lentiviral particles are generated from three main components:
The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.
Lentiviral particles are generated from three main components:
- The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
- The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells
- The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.
The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.
Application
Functional Genomics/Screening/Target Validation
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| concentration 100 ng/μL (in Tris-EDTA) | concentration - | concentration - | concentration ≥1x106 VP/ml (via p24 assay) |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| application(s) CRISPR | application(s) - | application(s) - | application(s) CRISPR |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SHP002-0.25ML | 04061838043719 |
| SHP002-1.7ML | 04061838248848 |