This product is free of EDTA and does not contain any preservatives. The final buffer formulation consists of 4.5 g/L sodium chloride, with 165 g/L sucrose added as a cryoprotectant. There is no glycerol or phosphate in the formulation.
SAE0053
Lipoprotein, low density from human plasma, solution
Synonym(s):
LDL, Low density lipoprotein, beta-lipoprotein
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About This Item
NACRES:
NA.61
UNSPSC Code:
12352202
Pricing and availability is not currently available.
Biochem/physiol Actions
Low-density lipoprotein (LDL) is a lipoprotein that carries cholesterol throughout the body via blood circulation. LDL has a role in dispositioning cholesterol to tissues and organs of the body. Colloquially deemed as the "bad cholesterol", having high levels of LDL can lead to plaque formation in arteries.
LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters, and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits; receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle, resulting in dissociation of LDL from its receptor. After loss of the clathrin coat, the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake
LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters, and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits; receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle, resulting in dissociation of LDL from its receptor. After loss of the clathrin coat, the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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| form liquid | form liquid | form solution | form solution |
| storage temp. −70°C | storage temp. −70°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| SAE0053-10MG | 04061831159981 |
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Does the product SAE0053-10MG contain EDTA or any antioxidant agent in its composition?
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1. Does the product have certification that it is free of endotoxins. 2. For dilution process is possible to use PBS with EDTA?
1 answer-
This product is not endotoxin tested. Currently, an endotoxin tested product option is not available. This solution may be diluted for use in PBS with EDTA. A similar product, L8292, is lyophilized from a solution containing 0.15 M NaCl and 0.01% EDTA. Note that EDTA may inhibit receptor binding at high concentrations.
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Working with SAE0053-10MG, lot 0000340421. The COA shows 10 mg but no volume or concentration. Could you confirm the exact volume (mL) and concentration (mg/mL) of this LDL solution?
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The volume and concentration for this product are not fixed. The total cholesterol content of one bottle is 10 mg. A typical batch has a concentration of 5,000 mg cholesterol/dL, which is equivalent to 50 mg cholesterol/mL. Therefore, the 10 mg of cholesterol would be in 0.2 mL of solution.
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When working with LDL (SAE0053), is it acceptable to store a working solution at 4°C if used within the same week for several treatments? Alternatively, can LDL go through freeze-thaw cycles?
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To thaw the product, it should be quickly thawed in a 37°C water bath and removed as soon as it is thawed. The product should then be stored at 2 – 8°C for up to one week, at -20°C for up to three months, and at -70°C for up to 24 months. For further dilutions, it should be diluted with 0.15 M sodium chloride.
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What is the difference between products SAE0053 and 437644 (both LDL from human plasma)? Can you provide more characetristics of SAE0053 product?
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Regarding Product SAE0053, the volume and concentration are not fixed, the only certainty is that the total cholesterol content of one bottle is 10mg. The total LDL particle concentration (protein+cholesterol+other lipids) is not currently determined, but based on published literature it is expected to be approximately twice the cholesterol concentration.
Please see the link below for testing performed on this product:
https://www.sigmaaldrich.cn/specification-sheets/228/747/SAE0053-10MG______SIGMA____.pdf
Regarding Product 437644, a 10 mg vial contains ~2 mg of protein. This product is under the product line Calbiochem®. The formulation is 150 mM NaCl, 0.01% EDTA, pH 7.4. The source of this material is prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.Please see the link below for testing performed on this product:
https://www.sigmaaldrich.com/specification-sheets/228/747/SAE0053-10MG______SIGMA____.pdfHelpful?
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