Following is the formulation of the inquired product: 0.01M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride.
P2272
Phosphate buffered saline
liquid, pH 7.2, sterile-filtered, suitable for cell culture
Synonym(s):
Phosphate buffered saline, PBS
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Product Name
Phosphate buffered saline, pH 7.2, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, tested
pH
7.2
General description
Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form tablet |
| pH 7.2 | pH 7.4 | pH 7.2 (25 °C) | pH 7.2-7.6 (1 tablet/200 mL), 7.4 |
| sterility sterile-filtered | sterility sterile-filtered | sterility autoclaved | sterility - |
| impurities Endotoxin, tested | impurities Endotoxin, tested | impurities - | impurities - |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
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I found a publication talking about 0.01 M saline phosphate buffer solution at pH8. I do not understand how to translate 1X PBS solution into molarities. Do you have a clue?
1 answer-
Helpful?
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What is the composition of P2272 Phosphate buffered saline pH 7.2, liquid, sterile-filtered, suitable for cell culture ?
1 answer-
The formulation of this Phosphate Buffered Saline product is as follows:
Potassium Phosphate Monobasic - 210 mg/L
Sodium Phosphate Dibasic, dihydrate - 482 mg/L
Sodium Chloride - 9.0 G/LHelpful?
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Is this calcium-magnesium free?
1 answer-
This PBS formulation does not contain Calcium or Magnesium.
Helpful?
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Is this PBS x1? Or x10? Not specified on the bottle
1 answer-
This product is a 1X formulation. Please see the link below to review available 10X PBS products:
https://www.sigmaaldrich.com/search/phosphate-buffered-saline-10x?focus=products&page=1&perpage=30&sort=relevance&term=Phosphate%20buffered%20saline%2010X&type=productHelpful?
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