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MAK437

Glutathione Peroxidase Assay Kit

Sufficient for 100 colorimetric tests

Synonym(s):

GPx Activity Assay Kit, GPx Activity Detection Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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Quality Segment

detection method

colorimetric

relevant disease(s)

cancer, diabetes; endocrinological disorders

storage temp.

−20°C

General description

Glutathione peroxidase (GPX) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell.

Application

The Glutathione Peroxidase Assay Kit may be used for:
  • Cancer Research
  • Diabetes Research
  • Oxidative Stress Research

The kit is suitable for the quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity in biological samples.

Biochem/physiol Actions

The Glutathione Peroxidase Assay Kit directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340 nm (A340) is directly proportional to the enzyme activity in the sample.

Features and Benefits

Enhanced Detection Range: Detect GPx activity accurately across a wide range, from 40 to 800 U/L, using only 10 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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This Item
CGP1GRSAMAK440
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

relevant disease(s)

cancer, diabetes; endocrinological disorders

relevant disease(s)

-

relevant disease(s)

-

relevant disease(s)

Alzheimer′s disease; Parkinson′s disease; cancer; neurological disorders; aging/geriatric diseases


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Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Org. Perox. F - Skin Corr. 1B - STOT RE 2 Inhalation - STOT SE 3

target_organs

Lungs, Respiratory system

Storage Class

5.2 - Organic peroxides and self-reacting hazardous materials

flash_point_f

174.2 °F

flash_point_c

79 °C



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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. What is assay buffer?

    1 answer
    1. The composition of the assay buffer is proprietary and not disclosed.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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