M7523

Anti-Myosin (Skeletal) antibody produced in rabbit

whole antiserum

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162704
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IHC (p)
• Citations: 31

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• species reactivity: human
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human or animal skeletal muscle, indirect immunofluorescence: 1:20 using human or animal sletetal muscle
• UniProt accession no.: P11055
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYH13(8735), MYH3(4621), MYL1(4632)

Description

General description

Myosin is a 480,000 dalton protein containing two identical heavy chains (200,000 daltons each) and four light chains (15,000-26,000 daltons). Myosin molecules consist of two major regions: tails (rods) and heads; they aggregate into filaments through the tail region. Multiple forms of myosin heavy chains exist for each muscle type; skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle.

Immunogen

whole myosin (heavy and light chains) from human skeletal muscle.

Application

Anti-Myosin (Skeletal) antibody produced in rabbit has been used in:

• immunohistochemistry
• immunostaining
• western blotting
• immunofluorescence
• immunolocalization
• immunocytochemistry[1]

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Specifically labels the A bands of human and animal skeletal muscle. Does not stain human smooth muscle tissue.

Analysis Note

The antiserum has been treated to remove lipoproteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable