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Lectin from Phaseolus vulgaris (red kidney bean)

Name: Lectin from <I>Phaseolus vulgaris</I> (red kidney bean)
Brand: SIGMA

Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

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About This Item

UNSPSC Code:

12352202

EC Number:

232-718-7

NACRES:

NA.32

Form:

lyophilized powder

Pricing and availability is not currently available.

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Properties

Quality Segment

200

product line

BioReagent

form

lyophilized powder

potency

<40 μg per mL agglutination activity

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

PBS: 2 mg/mL

storage temp.

2-8°C

Description

Application

Lectin from Phaseolus vulgaris (red kidney bean) is used in Lymphocyte Transformation Assay and culture medium.[1]

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

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Show Differences

1 of 1

This Item	L2646	L2769	L5380
Sigma-Aldrich L8902 Lectin from Phaseolus vulgaris (red kidney bean)	Sigma-Aldrich L2646 Lectin from Phaseolus vulgaris (red kidney bean)	Sigma-Aldrich L2769 Lectin from Phaseolus vulgaris (red kidney bean)	Sigma-Aldrich L5380 Lectin from Pisum sativum (pea)
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) -	technique(s) -
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
solubility PBS: 2 mg/mL	solubility -	solubility -	solubility -
impurities salt, free	impurities salt, free	impurities salt, free	impurities -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000524493

0000524494

0000524495

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Global Trade Item Number

SKU	GTIN
L8902-5MG	04061835557639
L8902-25MG	04061833966242
L8902-100MG	04061833966228

Questions

Anonymous

2 years ago

What do the designations -P, -L, -M, and -E represent in the names of PHA products?

1 answer
1. Technical Support
  
  2 years ago
  
  Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:
  1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.
  2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.
  PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.
  
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