The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
L7759
Lectin from Arachis hypogaea (peanut)
peroxidase conjugate, lyophilized powder
Synonym(s):
PNA, Peanut agglutinin
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| Pack Size | SKU | Availability | Price |
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About This Item
biological source
Arachis hypogaea (peanut)
Quality Segment
conjugate
peroxidase conjugate
form
lyophilized powder
peroxidase activity
≥20 units/mg protein
technique(s)
western blot: suitable
storage temp.
−20°C
Application
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
Physical form
Preparation Note
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) western blot: suitable | technique(s) - | technique(s) - | technique(s) western blot: suitable |
| biological source Arachis hypogaea (peanut) | biological source - | biological source - | biological source Ulex europaeus |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate peroxidase conjugate | conjugate FITC conjugate | conjugate - | conjugate peroxidase conjugate |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Product Information Sheet
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L7759-250UG | 04061833965672 |
| L7759-1MG | 04061835557608 |
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How does the storage temperature relate to shipping conditions?
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Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein? If so, what is the carbohydrate content?
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Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate. Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure". Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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