We have not studied buffers and solubility with this product. However, in checking in literature and as reported by various other vendors and the Chemical book, the solubility of this product, CAS # 149371-12-0, is in Water > 10 mg/mL.
Per other vendors, product may be soluble in PBS, pH 6.5
H6512
Heparinase II from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100 units/mg protein (enzyme + BSA)
Synonym(s):
Heparin lyase II
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About This Item
Specific activity:
≥100 units/mg protein (enzyme + BSA)
Biological source:
bacterial (Flavobacterium heparinum)
Pricing and availability is not currently available.
biological source
bacterial (Flavobacterium heparinum)
Quality Segment
conjugate
conjugate (Glucosaminoglycan)
form
lyophilized powder
specific activity
≥100 units/mg protein (enzyme + BSA)
mol wt
84.1 kDa
storage temp.
−20°C
General description
Heparinase II is one of three key heparin-degrading enzymes of Flavobacterium heparinum (also known as Pedobacter heparinus1). It belongs to the polysaccharide lyases family PL21.
Application
Heparinase II from Flavobacterium heparinum has been used:
- for digestion of heparin sulfate during exosome isolation
- for digestion of heparin sulfate in notochordal cell conditioned media (NCCM) to investigate the content of glycosaminoglycans in NCCM
- as a component of digestion buffer during cell surface glycan processing
- as a component of heparin lyase (HSase) mix to remove the heparin sulfate on the 293ͰT/ACE2 cell surface and study the effect of histones on the infectivity of pseudovirus
Biochem/physiol Actions
Heparinase II cleaves heparan sulfate, and to a lesser extent heparin (relative activity about 2:1), at the α (1-4) linkages between hexosamines and uronic acid residues (both glucuronic and iduronic), yielding mainly disaccharides. Heparinase II has the broadest substrate specificity of the three heparinases Heparinase enzymes helps in decoding the complex structures of substrates. Heparin interferes with DNA transcription in PCR and in reverse transcription of RNA. Heparinase II has been used to remove heparin for downstream analysis of genomic DNA.
Other Notes
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.0 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| biological source bacterial (Flavobacterium heparinum) | biological source bacterial (Flavobacterium heparinum) | biological source - | biological source bacterial (Flavobacterium heparinum) |
| specific activity ≥100 units/mg protein (enzyme + BSA) | specific activity ≥200 units/mg protein | specific activity ≥200 units/mg protein (enzyme + BSA) | specific activity ≥30 units/mg protein (enzyme + BSA) |
| form lyophilized powder | form lyophilized powder | form lyophilized solid | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 84.1 kDa | mol wt - | mol wt 42.8 kDa | mol wt 70.8 kDa |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The heparinase datasheets do not provide information on how to use them in a digestion reaction. I would like to use heparinase I, II, and III together. Which reaction buffer should I prepare? How many units of each heparinase should I add?
1 answer-
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Hi, in which solution should I dissolve this Heparinase II?
1 answer-
This product is soluble in most aqueous buffers. The suggested buffer for this material is 100 mM sodium acetate buffer containing 0.01% BSA, pH 7.0. A concentration of approximately 17-35 Units / mL is optimal. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/242/914/h6512pis.pdfHelpful?
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I purchased this product and attempted to dissolve it, but I couldn't find the composition of the storage buffer. Could you please provide the buffer composition?
1 answer-
The specific formulation of this product is considered proprietary. However, this enzyme is lyophilized from a Phosphate Buffer solution containing Bovine Serum Albumin as a stabilizer.
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