To make a 100 mM stock solution with 250 mg of β-Estradiol (molecular weight 272.38 g/mol), dissolve it in 9.18 mL of ethanol. This is calculated as (0.250 g / 272.38 g/mol) / 0.1 mol/L = 0.00918 L or 9.18 mL. This can be done directly in a clean, dry glass bottle large enough to hold and mix the solution.
E2758
β-Estradiol
BioReagent, powder, suitable for cell culture
Synonym(s):
1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
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About This Item
Empirical Formula (Hill Notation):
C18H24O2
CAS Number:
Molecular Weight:
272.38
UNSPSC Code:
12352209
NACRES:
NA.77
PubChem Substance ID:
EC Number:
200-023-8
Beilstein/REAXYS Number:
1914275
MDL number:
Biological source:
synthetic (organic)
Form:
powder
Assay:
≥98% (HPLC)
Pricing and availability is not currently available.
biological source
synthetic (organic)
Quality Level
product line
BioReagent
assay
≥98% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
mp
176-180 °C (lit.)
solubility
ethanol: 50 mg/mL, clear, colorless
shipped in
ambient
storage temp.
room temp
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This Item | |||
|---|---|---|---|
| biological source synthetic (organic) | biological source synthetic (organic) | biological source - | biological source - |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay - | assay - |
| product line BioReagent | product line BioXtra | product line - | product line - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) - |
| form powder | form powder | form - | form - |
General description
β-Estradiol or estradiol-17 β (E2) is secreted majorly by the large preovulatory follicles in the ovary. In addition, estradiol can be synthesized de novo from cholesterol have androgens synthesized from cholesterol. The aromatization of androgen by enzyme aromatase (P450arom) results in the β-estradiol production.
Application
β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).
β-Estradiol has been used:
- as a component of Dulbecco′s modified Eagle medium (DMEM) for culturing mammary tumor cells
- as a oestrogenic compound for testing neuroprotective effects
- as a component of medium199 for culturing oocytes
Biochem/physiol Actions
The major estrogen secreted by the premenopausal ovary.
The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.
β-Estradiol (E2) is a major premenopausal hormone in women and is essential for spermiogenesis, sperm maturation and motility in men. Cultured bone marrow stem cells (BMSCs) have ability to synthesize E2 and could be exploited for treating estrogen deficiency. It mediates physiological functions through estrogen receptor (ER) α and ERβ.
Physical form
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Preparation Note
To prepare a 20 μg/ml stock solution, add 1 ml absolute ethanol to 1 mg β-estradiol; gently swirl to dissolve; add 49 ml sterile medium while mixing.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Estrogen synthesis and signaling pathways during aging: from periphery to brain
Cui J, et al.
Trends in Molecular Medicine, 19(3), 197-209 (2013)
Selective oestrogen receptor (ER) modulators reduce microglia reactivity in vivo after peripheral inflammation: potential role of microglial ERs
Tapia-Gonzalez S, et al.
The Journal of Endocrinology, 198, 219?230-219?230 (2008)
The developmental potential of oocytes is impaired in cattle with liver abnormalities
Sarentonglaga B, et al.
Journal of Reproduction and Development, 59(2), 168-173 (2013)
Estradiol synthesis and release in cultured female rat bone marrow stem cells
Zhang D, et al.
BioMed Research International, 2013 (2013)
Loss of STAT1 from mouse mammary epithelium results in an increased Neu-induced tumor burden
Klover PJ, et al.
Neoplasia, 12(11), 899-905 (2010)
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E2758-250MG | 04061835568390 |
| E2758-5G | 04061835514137 |
| E2758-1G | 04061835568383 |
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Hello, you mention reconstitution in ug/ml. How much ethanol do I need to dissolve 250mg in to get a 100mM stock solution? Would I be able to do it directly in the glass bottle?
1 answer-
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Can Estradiol/ethanol solutions be sterile filtered?
1 answer-
Yes. stock solutions in ethanol may be sterile filtered. However, cellulose-based filter membranes are not recommended for use with ethanol. Please see the link below to review additional information regarding filter membrane compatibility with commonly used solvents:
https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/chemical-compatibility-of-filter-componentsHelpful?
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Hello, I would like to check the solubility of this product in DMSO. Best,
1 answer-
The solubility of this product in DMSO has not been determined. However, as per the literature, the β-Estradiol is soluble in DMSO at 20 mg/ml.
Helpful?
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after dissolving the β-Estradiol in ethanol , whats the tempreture to store the item in ?
1 answer-
The storage or stability conditions once the compound is in solution is not determined. General recommendations for β-Estradiol are to protect from light, and store at -20C or at 2-8C in a degassed solution. Solutions stored in this manner should remain stable in the range of 1 month - 1 year.
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How diluit for concentration of the 100ng/mL
1 answer-
β-Estradiol is soluble in absolute ethanol at 50 mg/mL (See E8875). Preparing a 100 ng/mL solution would require serial dilutions.
Start with a 1 mg/mL (1000 ug/mL) stock in ethanol.
Dilute 10 uL (10 ug = 10,000 ng) of this stock with 9.990 mL to achieve a 1000 ng/mL solution. This can be diluted with medium or buffer.
Dilute 1 mL of this with 9 mL to the final concentration of 100 ng/mL.Helpful?
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