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D8273

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA

lyophilized powder

Synonym(s):

single-stranded DNA-Cellulose, ss-DNA-Cellulose, DNA-Cellulose

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About This Item

UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51
MDL number:
MFCD00130925
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C
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biological source

bovine (calf) thymus

Quality Segment

200

form

lyophilized powder

extent of labeling

≥3 mg single-stranded calf thymus DNA/g

storage temp.

−20°C

General description

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.[1]

Application

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.

Analysis Note

May contain a trace of Tris and EDTA.

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Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA lyophilized powder

Sigma-Aldrich

D8273

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA lyophilized powder

Sigma-Aldrich

D8515

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Sigma-Aldrich

D8899

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Deoxyribonucleic acid from calf thymus Genomic, unsheared

Sigma-Aldrich

D4764

Deoxyribonucleic acid from calf thymus

biological source

bovine (calf) thymus

biological source

bovine (calf) thymus

biological source

bovine (calf) thymus

biological source

bovine (calf) thymus

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

extent of labeling

≥3 mg single-stranded calf thymus DNA/g

extent of labeling

3-8 mg double-stranded calf thymus DNA/g

extent of labeling

-

extent of labeling

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000565780
0000565780
0000499240
0000499239
0000499243

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Global Trade Item Number

SKUGTIN
D8273-5G04061833662755
D8273-1G04061826690659
D8273-10G04061833589342

Questions

  1. Instructions on how to pack a ssDNA cellulose column, like how to soak the powder (buffer, for how long, and temperature). Thanks

    1 answer

    1. The powder may be reconstituted in 10 mM Tris HCl buffer (pH 7.4) containing 1 mM EDTA. One gram of powder will swell to roughly 3 milliliters of resin.
      Relatively high volumes are required to maintain a suspension; a range of 15 – 30 mL per gram of dry powder is typical.
      For additional buffer recommendations, please refer to Methods in Enzymology, volume XXI, page 199 (1971).
      Gravity flow through a disposable column is recommended for best results.
      The use of a pump may cause the cellulose to compress, resulting in high back pressure and a very slow flow rate.

      Please see the research papers linked below that describe the use of this product in purification applications.

      https://journals.asm.org/doi/pdf/10.1128/mcb.16.10.5604
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316966/

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