D6293
pUC18 DNA Hae III Digest
for DNA electrophoresis
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About This Item
NACRES:
NA.25
UNSPSC Code:
12352202
Pricing and availability is not currently available.
General description
Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.
Preparation Note
Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
Contains 11 fragments, 11–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5%.
Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
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This Item | |||
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| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
| usage 200 uses (0.1 mg size), 40 uses (20 μg size) | usage 200 uses (0.1 mg size), 40 uses (20 μg size) | usage 100 uses (0.1 mg size), 500 uses (0.5 mg size) | usage 1000 uses (0.5 mg size), 200 uses (0.1 mg size) |
| Quality Level 100 | Quality Level 100, 200 | Quality Level 100, 200 | Quality Level 100 |
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Development of competitive PCR for detection of Butyrivibrio fibrisolvens in the rumen
Mrazek J and Kopecny J
Folia Microbiologica, 46(1), 63-65 (2001)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D6293-.1MG | 04061833587478 |
| D6293-20UG | 04061833587485 |