CS0780

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

• Synonym(s): Beta-NAG Activity Assay Kit
• UNSPSC Code: 12161503
• EC Number: 222-398-7
• NACRES: NA.84

Properties

• Quality Segment: 200
• usage: sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... NAGLU(4669)

Description

Application

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

Biochem/physiol Actions

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

Analysis Note

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

Other Notes

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Kit Components Only

• Dilution Buffer 8 mL
• 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
• Citrate Buffer Solution, 0.09 M 100 mL
• p-Nitrophenol Standard Solution, 10 mM 1 mL
• β-N-Acetylglucosaminidase from Jack beans 1 vial
• Sodium carbonate 5 g

Safety Information

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H319,H351,H373
• pcodes: P202 - P260 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
• target_organs: Liver,Kidney
• wgk: WGK 3
• Storage Class: 10 - Combustible liquids