C3545

Monoclonal Anti-Calmodulin antibody produced in mouse

clone 6D4, ascites fluid

• Synonym(s): Anti-CALM3, Anti-calmodulin
• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD00162206
• Conjugate: unconjugated
• Clone: 6D4, monoclonal
• Application: WB
• Citations: 13

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 6D4, monoclonal
• contains: 15 mM sodium azide as preservative
• species reactivity: chicken, Dictyostelium discoideum, bovine, rat
• technique(s): western blot: 1:2,000 using a bovine brain calmodulin preparation
• isotype: IgG1
• UniProt accession no.: P0DP29
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Calm1(24242)

Description

General description

Calmodulin belongs to a family of structurally homologous Ca²⁺-binding proteins. CALM1 gene is located on human chromosome 14q32.11.

Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.

Immunogen

preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

Application

Monoclonal Anti-Calmodulin antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblot
• dot blot
• immunocytochemistry
• immunoprecipitation
• immunofluorescence[1]

Biochem/physiol Actions

Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes.

The antibody recognizes native and SDS-denatured calmodulin.

These antibodies are directed against different epitopes within the calmodulin molecule.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable