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C-34006

Calcium Chloride Solution

CaCl 2-Solution (0.5 M), 2 ml

Synonym(s):

Calcium Chloride Liquid

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About This Item

NACRES:
NA.81
UNSPSC Code:
12352207
Form:
solution
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form

solution

packaging

pkg of 2 ml

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

General description

CaCl 2-Solution

Application

Some types of primary human cells, especially Normal Human Epidermal Keratinocytes (NHEK), respond sensitively to the calcium concentration in the culture medium. Calcium Chloride solution allows the adjustment of the optimal amount of calcium required by the cells. PromoCell Calcium Chloride (CaCl2) Solution is prepared as a solution from highly pure tissue culture tested Calcium Chloride and cell culture-grade water. It is a clear colorless and sterile solution.The concentration of CaCl2 is 0.5 M.

Preparation Note

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Store at -20°C in the dark immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label.

Analysis Note

All lots of PromoCell Calcium Chloride Solution are subjected to comprehensive quality control tests. Each lot is routinely tested for the use with human primary cells, absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).

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This Item
21115C5670449709
form

solution

form

liquid

form

powder

form

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | plant: suitable

technique(s)

-

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

packaging

pkg of 2 ml

packaging

-

packaging

-

packaging

-

storage temp.

−20°C

storage temp.

-

storage temp.

-

storage temp.

-


Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–3 of 3 Questions  
  1. Is this a hexahydrate? I was wondering if the cacl2 is in solution as a hexahydrate?

    1 answer
    1. Product C-34006 is manufactured by Promocell. MilliporeSigma has no information on record that this product is a hexahydrate. This information is not listed on the Promocell website. Please contact Promocell Tech Service for this inquiry: www.promocell.com.

      Helpful?

  2. Should it be thawed and re-frozen each time it is used?

    1 answer
    1. Store this product at -20°C in the dark immediately after arrival. To enhance its shelf-life, following the initial thaw, store it in small one-use-size aliquots in a none-frost-free freezer, as well as avoid multiple freeze/thaw cycles.

      Helpful?

  3. I received this product for my master assay, and I saved it in -20 C. What should i do in order to use it and to change its concentration? Thank you so much for your time,

    1 answer
    1. Unfortunately, a protocol for dilution is not available. However, the following information from the product manual may be helpful. Some types of primary human cells, especially Normal Human Epidermal Keratinocytes, respond sensitively to the calcium concentration in the culture medium. Calcium Chloride solution allows the adjustment of the optimal amount of calcium required by the cells. The Calcium Chloride (CaCl2) Solution is prepared as a solution from highly pure tissue culture tested Calcium Chloride and cell culture-grade water. It is a clear, colorless, and sterile solution. The concentration of CaCl2 is 0.5 M, and each vial contains 2 mL.

      Helpful?

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