C0733
Cyclooxygenase 1 from sheep
glycerol solution, ≥1500 units/mg protein
Synonym(s):
COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase
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About This Item
Specific activity:
≥1500 units/mg protein
Pricing and availability is not currently available.
form
glycerol solution
Quality Level
specific activity
≥1500 units/mg protein
mol wt
dimer subunit mol wt 70 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
sheep ... COX1(808251)
General description
Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain. Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.
Application
Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples and in prostaglandin synthase activity assay.
Biochem/physiol Actions
COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent KM of 8.3 μM for arachidonic acid.
Physical form
Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN® 20, 300 μM diethyldithiocarbamate and 10% glycerol.
Other Notes
One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.
Legal Information
TWEEN is a registered trademark of Croda International PLC
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This Item | |||
|---|---|---|---|
| Gene Information sheep ... COX1(808251) | Gene Information human ... PTGS2(5743) | Gene Information human ... COX1(6775083) | Gene Information human ... COX2(6775079) |
| specific activity ≥1500 units/mg protein | specific activity ≥8000 units/mg protein | specific activity - | specific activity - |
| form glycerol solution | form aqueous solution | form frozen liquid | form frozen liquid |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| mol wt dimer subunit mol wt 70 kDa | mol wt dimer subunit mol wt 70-74 kDa | mol wt 70.5 kDa | mol wt 71 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Michela Zago et al.
Toxicology letters, 226(2), 107-116 (2014-01-30)
Diseases due to cigarette smoke exposure, including chronic obstructive pulmonary disease (COPD) and lung cancer, are associated with chronic inflammation typified by the increased expression of cyclooxygenase-2 (COX-2) protein. RelB is an NF-κB family member that suppresses cigarette smoke induction
Sze Chern Lim et al.
American journal of human genetics, 94(2), 209-222 (2014-01-28)
Leigh syndrome (LS) is a severe neurodegenerative disorder with characteristic bilateral lesions, typically in the brainstem and basal ganglia. It usually presents in infancy and is genetically heterogeneous, but most individuals with mitochondrial complex IV (or cytochrome c oxidase) deficiency
S Ohki et al.
The Journal of biological chemistry, 254(3), 829-836 (1979-02-10)
The highly purified prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes had two still unresolved enzyme activities; the oxygenative cyclization of 8,11,14-eicosatrienoic acid to produce prostaglandin G1 and the conversion of the 15-hydro-peroxide of prostaglandin G1 to a 15-hydroxyl group
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0733-5000UN | 04061833451533 |